Characterisation of the gene encoding suilysin from Streptococcus suis and expression in field strains

The gene encoding suilysin was cloned from Streptococcus suis serotype 2 strain P1/7. Analysis of the nucleotide and translated amino acid sequence confirmed suilysin to be a member of the thiol activated cytolysin group (TACY). The pneumolysin from Streptococcus pneumoniae is the most closely relat...

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Bibliographic Details
Published in:FEMS microbiology letters 1998-10, Vol.167 (2), p.255-261
Main Authors: Segers, R.P.A.M, Kenter, T, Haan, A.M. de, Jacobs, A.A.C
Format: Article
Language:English
Subjects:
Amino acid sequence
Amino acids
animal diseases
animal health
Animals
Antibodies, Monoclonal
bacterial infections
Bacteriology
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA, Bacterial - analysis
E coli
Escherichia coli
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
genbank/z36907
Gene expression
Genes, Bacterial
Hemolysin Proteins - genetics
Hemolysin Proteins - metabolism
Hemolysis
human health and safety
medicine
Microbiology
Molecular Sequence Data
Nucleotides
Organic Chemicals
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phylogeny
Pneumolysin
Polymerase Chain Reaction - methods
Recombinant Proteins - biosynthesis
Sequence Analysis, DNA
Serotyping
Sly gene
Streptococcus infections
Streptococcus suis
Streptococcus suis - classification
Streptococcus suis - genetics
Suilysin
Thiol‐activated cytolysin
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Summary:The gene encoding suilysin was cloned from Streptococcus suis serotype 2 strain P1/7. Analysis of the nucleotide and translated amino acid sequence confirmed suilysin to be a member of the thiol activated cytolysin group (TACY). The pneumolysin from Streptococcus pneumoniae is the most closely related orthologous gene known. Suilysin was overexpressed in E. coli in an active haemolytic form. A strong correlation between the presence of the sly gene and haemolytic activity in the supernatant of S. suis field strains was found. Of 158 strains tested, 63% contained the gene. Within the (most prevalent) serotype 2, the sly gene was demonstrated in 95% of the strains isolated in Eurasia, but only in 7% of the strains from North America.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1998.tb13236.x