Cloning and expression of the pediocin operon in Streptococcus thermophilus and other lactic fermentation bacteria

Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed...

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Bibliographic Details
Published in:Current microbiology 1999-11, Vol.39 (5), p.295-301
Main Authors: Coderre, P.E, Somkuti, G.A
Format: Article
Language:English
Subjects:
Bacteria
Bacteriocins - biosynthesis
Bacteriocins - genetics
Bacteriocins - pharmacology
Bacteriology
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Cloning
Cloning, Molecular
Dairy products
E coli
Enterococcus faecalis - genetics
Enterococcus faecalis - metabolism
Escherichia coli - genetics
Escherichia coli - metabolism
Fermentation
food preservatives
Fundamental and applied biological sciences. Psychology
Genetics
Lactobacillus - drug effects
Lactococcus lactis - genetics
Lactococcus lactis - metabolism
Microbial Sensitivity Tests
Microbiology
Mission oriented research
Operon
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Pediococcus - genetics
Pediococcus - metabolism
Plasmids - genetics
Preservatives
Streptococcus - drug effects
Streptococcus - genetics
Streptococcus - metabolism
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Summary:Production of pediocin in Pediococcus acidilactici is associated with pMBR1.0, which encodes prepediocin, a pediocin immunity protein, and two proteins involved in secretion and precursor processing. These four genes are organized as an operon under control of a single promoter. We have constructed shuttle vectors that contain all four structural genes, the chromosomal promoter ST(P2201) from Streptococcus thermophilus, and repA from the 2-kbp S. thermophilus plasmid pER8. The recombinant plasmid, pPC318, expressed and secreted active pediocin in Escherichia coli. Streptococcus thermophilus, Lactococcus lactis subsp. lactis, and Enterococcus faecalis were electrotransformed with pPC418, a modified vector fitted with an erythromycin resistance tracking gene. Pediocin was produced and secreted in each of the lactic acid bacteria, and production was stable for up to ten passages. The expression of pediocin in dairy fermentation microbes has important implications for bacteriocins as food preservatives in dairy products.
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900462