Pathogenicity of Salmonella enteritidis phage types 4, 8, and 23 in broiler chicks

• Published in: Avian diseases 1999-07, Vol.43 (3), p.506-515
• Main Authors: Dhillon, A.S, Alisantosa, B, Shivaprasad, H.L, Jack, O, Schaberg, D, Bandli, D
• Format: Article
• Language: English
• Subjects: Animals; Bacteriophages; Body Weight; Chickens; Chicks; Colony-Forming Units Assay; Diseases; Hepatitis, Animal - pathology; histopathology; Humans; Inflammation; Liver; Liver - pathology; Mortality; Myocarditis - pathology; Myocarditis - veterinary; pathogenicity; Pericarditis - pathology; Pericarditis - veterinary; Poultry Diseases - microbiology; Poultry Diseases - pathology; Poultry Diseases - physiopathology; Salmonella; Salmonella - isolation & purification; Salmonella - pathogenicity; Salmonella enterica subsp. enterica serovar Pullorum; Salmonella enteritidis; Salmonella enteritidis - isolation & purification; Salmonella enteritidis - pathogenicity; Salmonella enteritidis - virology; Salmonella Infections, Animal - pathology; Salmonella Infections, Animal - physiopathology; Salmonella Phages - pathogenicity; Virulence; Yolk sac
• ISSN: 0005-2086; 1938-4351
• DOI: 10.2307/1592649

Four hundred fifty day-old Hubbard broiler chicks were subdivided into 15 groups of 30 chicks each. Six groups of chicks received 0.5 ml of broth culture containing 5 x 10(6) colony-forming units (CFU) of Salmonella enteritidis (SE) phage types (PTs) 4, 8, and 23 by crop gavage. Similarly, six other groups received 0.5 ml containing 5 x 10(8) CFU of SE. One group was inoculated with 0.5 ml containing 5 x 10(6) CFU of Salmonella pullorum, and another group received 0.5 ml containing 5 x 10(8) CFU of S. pullorum. A group of 30 chicks were kept as uninoculated controls. Chicks were observed daily for clinical signs and mortality. All birds were weighed at 7, 14, and 21 days postinoculation 21 (DPI). Four chicks were randomly selected from each treatment group, euthanatized, and necropsied at 7 and 14 DPI. Gross lesions were recorded and selected tissues were collected for histopathology. The higher rates of illness and mortality were observed in chicks inoculated with 5 x 10(6) and 5 x 10(8) CPU of S. pullorum, followed by SE PT4 of human origin and SE PT4 of chicken origin. Moderate to high mortality was observed in chicks inoculated with the higher dose of SE isolates that belonged to PT8 and one SE of PT23. Variable mortality was evident in groups inoculated with the lower dose of salmonella. The most consistent gross and histopathologic changes, including fibrinous pericarditis and perihepatitis, were seen in the dead birds from various treatment groups. The lower mean body weights were present in all treatment groups compared with uninoculated controls. No illness or mortality was observed in uninoculated control groups.