Analysis of Gamma-Hydroxybutyrate (GHB) in Urine by Gas Chromatography-Mass Spectrometry

A simple method for the direct analysis of gamma-hydroxybutyrate (GHB) from human urine is described. The method uses solid-phase extraction, liquid-liquid extraction, and silyl-derivatization, then gas chromatographic-mass spectrometric analysis using GHB-d6 as the internal standard. The method was...

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Bibliographic Details
Published in:Journal of analytical toxicology 1999-09, Vol.23 (5), p.301-305
Main Authors: McCusker, Rachel R., Paget-Wilkes, Helen, Chronister, Chris W., Goldberger, Bruce A., ElSohly, Mahmoud A.
Format: Article
Language:English
Subjects:
4-Butyrolactone - chemistry
4-Butyrolactone - urine
Analysis
Biological and medical sciences
Evaluation Studies as Topic
g-Hydroxybutyrate
Gas Chromatography-Mass Spectrometry - methods
General pharmacology
Humans
Medical sciences
Pharmacology. Drug treatments
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Sodium Oxybate - chemistry
Sodium Oxybate - urine
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Description
Summary:A simple method for the direct analysis of gamma-hydroxybutyrate (GHB) from human urine is described. The method uses solid-phase extraction, liquid-liquid extraction, and silyl-derivatization, then gas chromatographic-mass spectrometric analysis using GHB-d6 as the internal standard. The method was linear from 5 to 500 mg/L, and coefficients of variation were less than 10%. Twenty-six urine specimens previously analyzed by an existing method were analyzed and yielded GHB concentrations ranging from 0 to 6100 mg/L; the results correlated between the two methods. Compared with existing methods, the method described here is superior because it is specific to GHB and can discriminate between GHB and gamma-butyrolactone.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/23.5.301