EFFECTS OF LIPID MEDIATORS ON THE SYNTHESIS OF LEUKAEMIA INHIBITORY FACTOR AND INTERLEUKIN 6 BY HUMAN BONE MARROW STROMAL CELLS

Human bone marrow stromal cells regulate haematopoiesis by releasing cytokines such as leukaemia inhibitory factor (LIF) and interleukin 6 (IL-6). We have investigated the effects of 5 lipid mediators (i.e. 12-HETE, 15-HETE, platelet-activating factor (PAF), LTB4and prostaglandin E2(PGE2)) on their...

Full description

Saved in:
Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Pa.), 1998-10, Vol.10 (10), p.781-785
Main Authors: Denizot, Y., Trimoreau, F., Praloran, V.
Format: Article
Language:English
Subjects:
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - pharmacology
Bone Marrow Cells - metabolism
Cells, Cultured
Culture Media, Serum-Free
Dinoprostone - pharmacology
Enzyme-Linked Immunosorbent Assay
Growth Inhibitors - biosynthesis
Humans
Hydroxyeicosatetraenoic Acids - pharmacology
Interleukin-1 - pharmacology
Interleukin-6 - biosynthesis
Leukemia Inhibitory Factor
Leukotriene B4 - pharmacology
Lipids - pharmacology
Lymphokines - biosynthesis
PAF/prostaglandin/leukotriene/HETE/LIF/IL-6/marrow stromal cells
Platelet Activating Factor - pharmacology
Stromal Cells - metabolism
Tumor Necrosis Factor-alpha - pharmacology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human bone marrow stromal cells regulate haematopoiesis by releasing cytokines such as leukaemia inhibitory factor (LIF) and interleukin 6 (IL-6). We have investigated the effects of 5 lipid mediators (i.e. 12-HETE, 15-HETE, platelet-activating factor (PAF), LTB4and prostaglandin E2(PGE2)) on their LIF and IL-6 synthesis. 12-HETE, 15-HETE and LTB4(both at 1 μM) stimulate the LIF production by human bone marrow stromal cells grown in 10% fetal calf serum (FCS). In contrast, PAF and PGE2have no effect. 12-HETE (1 μM) enhances LIF synthesis in serum free medium 7.7-fold and stimulates IL-1 induced LIF production. 12-HETE, 15-HETE, PAF and LTB4have no effect on the spontaneous, serum- and cytokine-induced IL-6 synthesis by bone marrow stromal cells. In contrast PGE2significantly stimulates serum-induced IL-6 synthesis. This study reports for the first time that lipid mediators may act on human haematopoiesis by modulating LIF and IL-6 synthesis by bone marrow stromal cells. Particularly, 12-HETE enhances LIF but not IL-6 synthesis. The different regulation of IL-6 and LIF synthesis in response to lipid mediators highlights the complexity of the cytokine regulation into the human bone marrow.
ISSN:1043-4666
1096-0023
DOI:10.1006/cyto.1998.0360