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5-( N-Ethyl- N-isopropyl)-amiloride inhibits amino acid release from the ischemic rat cerebral cortex: role of Na +–H + exchange

The effect of the selective Na +/H + antiporter inhibitor 5-( N-ethyl- N-isopropyl)-amiloride (EIPA) on amino acid release from the ischemic/reperfused rat cerebral cortex was investigated using a cortical cup technique. EIPA (25 μM in artificial cerebrospinal fluid), applied topically, inhibited th...

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Published in:	Brain research 1998-11, Vol.812 (1), p.297-300
Main Authors:	Phillis, John W, O'Regan, Michael H, Song, Dekun
Format:	Article
Language:	English
Subjects:	5-( N-Ethyl- N-isopropyl)-amiloride Acidosis Amiloride Amiloride - analogs & derivatives Amiloride - pharmacology Amino acid Amino Acids - metabolism Animals Biological and medical sciences Brain Ischemia - drug therapy Cerebral ischemia Glutamate Male Medical sciences Na +/H + exchange Neurology Rats Rats, Sprague-Dawley Sodium-Hydrogen Exchangers - antagonists & inhibitors Vascular diseases and vascular malformations of the nervous system
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container_title	Brain research
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creator	Phillis, John W O'Regan, Michael H Song, Dekun
description	The effect of the selective Na +/H + antiporter inhibitor 5-( N-ethyl- N-isopropyl)-amiloride (EIPA) on amino acid release from the ischemic/reperfused rat cerebral cortex was investigated using a cortical cup technique. EIPA (25 μM in artificial cerebrospinal fluid), applied topically, inhibited the ischemia–reperfusion evoked efflux of aspartate, glutamate, γ-aminobutyric acid, taurine and phosphoethanolamine. Reductions in the ischemia-evoked releases of these amino acids suggest that ischemia precipitates acidosis, Na +/H + exchange and cell swelling with amino acid release as the cells mount a regulatory volume decrease response. EIPA, by blocking Na +/H + exchange, would reduce cell swelling and the resulting amino acid release.
doi_str_mv	10.1016/S0006-8993(98)00984-6
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source	ScienceDirect Freedom Collection 2022-2024
subjects	5-( N-Ethyl- N-isopropyl)-amiloride Acidosis Amiloride Amiloride - analogs & derivatives Amiloride - pharmacology Amino acid Amino Acids - metabolism Animals Biological and medical sciences Brain Ischemia - drug therapy Cerebral ischemia Glutamate Male Medical sciences Na +/H + exchange Neurology Rats Rats, Sprague-Dawley Sodium-Hydrogen Exchangers - antagonists & inhibitors Vascular diseases and vascular malformations of the nervous system
title	5-( N-Ethyl- N-isopropyl)-amiloride inhibits amino acid release from the ischemic rat cerebral cortex: role of Na +–H + exchange
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