An autopsy case of malignant mesothelioma with osseous and cartilaginous differentiation bone morphogenetic protein-2 in mesothelial cells and its tumor

An autopsy case of biphasic malignant mesothelioma with osseous and cartilaginous differentiation diffusely involving the peritoneal cavity was confirmed by light microscopic histochemistry, immunohistochemistry, and electron microscopy. A reverse transcription-polymerase chain reaction using specif...

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Bibliographic Details
Published in:Digestive diseases and sciences 1999-08, Vol.44 (8), p.1626-1631
Main Authors: KIYOZUKA, Y, MIYAZAKI, H, YOSHIZAWA, K, SENZAKI, H, YAMAMOTO, D, INOUE, K, BESSHO, K, OKUBO, Y, KUSUMOTO, K, TSUBURA, A
Format: Article
Language:English
Subjects:
Abdominal Neoplasms - diagnostic imaging
Abdominal Neoplasms - metabolism
Abdominal Neoplasms - pathology
Aged
Biological and medical sciences
Bone and Bones - pathology
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins - genetics
Bone Morphogenetic Proteins - metabolism
Cartilage - pathology
Epithelium - metabolism
Epithelium - pathology
Fatal Outcome
Humans
Immunohistochemistry
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Mesothelioma - diagnostic imaging
Mesothelioma - metabolism
Mesothelioma - pathology
Osteoarticular system. Muscles
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
RNA, Messenger - metabolism
Tomography, X-Ray Computed
Transforming Growth Factor beta
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Summary:An autopsy case of biphasic malignant mesothelioma with osseous and cartilaginous differentiation diffusely involving the peritoneal cavity was confirmed by light microscopic histochemistry, immunohistochemistry, and electron microscopy. A reverse transcription-polymerase chain reaction using specific primers for bone morphogenetic protein-2 (BMP-2) revealed weak positive transcript in normal mesothelial cells and up-regulated expression around bone-forming malignant mesothelioma tissue. However, BMP-2 protein expression was detected only in the marginal zone of bone trabeculae and spindle-shaped mesothelioma cells distributed around bone trabeculae in tumor tissue. The distribution of type IV collagen in tumor tissue was in accordance with the BMP-2 expression. Normal mesothelial cells and tumor cells expressed BMP-2 mRNA, but the BMP-2 protein expression was restricted to the bone-forming area in the malignant mesothelioma.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1026627413715