Overexpression of SOX15 Inhibits Proliferation of NT2/D1 Cells Derived from a Testicular Embryonal Cell Carcinoma

SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2...

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Published in:Molecules and cells 2007-12, Vol.24 (3), p.323-328
Main Authors: Yan, Hong-Tao (The University of Tokushima, Tokushima, Japan), Shinka, Toshikatsu (The University of Tokushima, Tokushima, Japan), Sato, Youichi (The University of Tokushima, Tokushima, Japan), Yang, Xin-Jun (The University of Tokushima, Tokushima, Japan), Chen, Gang (The University of Tokushima, Tokushima, Japan), Sakamoto, Kozue (The University of Tokushima, Tokushima, Japan), Kinoshita, Keigo (The University of Tokushima, Tokushima, Japan), Aburatani, Hiroyuki (University of Tokyo, Tokyo, Japan), Nakahori, Yutaka (The University of Tokushima, Tokushima, Japan), E-mail: nakahori@basic.med.tokushima-u.ac.jp
Format: Article
Language:English
Subjects:
Carcinoma, Embryonal - pathology
CELL CYCLE
Cell Line, Tumor
cell proliferation
Cell Proliferation - drug effects
CICLO CELULAR
CYCLE CELLULAIRE
DNA-Binding Proteins - biosynthesis
Flow Cytometry
Gene Expression
High Mobility Group Proteins - biosynthesis
Humans
Male
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
SOX
SOX Transcription Factors
SOX15
Testicular Neoplasms - pathology
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Summary:SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were upregulated in the SOX15-NT2/Dl cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle.
ISSN:1016-8478
DOI:10.1016/s1016-8478(23)07346-6