Separation and identification of various carotenoids by C30 reversed-phase high-performance liquid chromatography coupled to UV and atmospheric pressure chemical ionization mass spectrometric detection

In this paper the application of on-line HPLC–UV–APCI (atmospheric pressure chemical ionization) mass spectrometry (MS) coupling for the separation and determination of different carotenoids as well as cis/trans isomers of β-carotene is reported. All HPLC separations were carried out under RP condit...

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Bibliographic Details
Published in:Journal of Chromatography A 1999-08, Vol.854 (1-2), p.37-44
Main Authors: Lacker, T, Strohschein, S, Albert, K
Format: Article
Language:English
Subjects:
Analytical chemistry
Atmospheric Pressure
Biological and medical sciences
Carotenoids
Carotenoids - analysis
Carotenoids - isolation & purification
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - methods
Exact sciences and technology
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Other chromatographic methods
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Terpenes
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Summary:In this paper the application of on-line HPLC–UV–APCI (atmospheric pressure chemical ionization) mass spectrometry (MS) coupling for the separation and determination of different carotenoids as well as cis/trans isomers of β-carotene is reported. All HPLC separations were carried out under RP conditions on self-synthesized polymeric C30 phases. The analysis of a carotenoid mixture containing astaxanthin, canthaxanthin, zeaxanthin, echinenone and β-carotene by HPLC–APCI-MS was achieved by scanning the mass range from m/z 200 to 700. For the characterization of a sample containing cis/trans isomers of β-carotene as well as their oxidation products, a photodiode-array UV–visible absorbance detector was used in addition between the column and the mass spectrometer for structural elucidation of the geometrical isomers. The detection limit for β-carotene in positive-ion APCI–MS was determined to be 1 pmol. In addition, an extract of non-polar substances in vegetable juice has been analyzed by HPLC–APCI-MS. The included carotenoids could be identified by their masses and their retention times.
ISSN:0021-9673
DOI:10.1016/S0021-9673(99)00584-1