Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso

African animal trypanosomoses constitute the most important vector-borne cattle diseases in sub-Saharan Africa. Generally it is considered that there is a great lack of accurate tools for the diagnosis of the disease. During a trypanosomosis survey in the agro-pastoral zone of Sideradougou, Burkina...

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Bibliographic Details
Published in:Veterinary parasitology 1999-09, Vol.86 (2), p.95-103
Main Authors: Solano, P, Michel, J.F, Lefrançois, T, de La Rocque, S, Sidibé, I, Zoungrana, A, Cuisance, D
Format: Article
Language:English
Subjects:
Animals
BOVIN
BURKINA FASO
Burkina Faso - epidemiology
CATTLE
Cattle-protozoa
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
DNA Primers - chemistry
DNA, Protozoan - chemistry
Electrophoresis, Agar Gel - veterinary
GANADO BOVINO
Hematocrit - veterinary
Microscopy - veterinary
PCR
Polymerase Chain Reaction - veterinary
Prevalence
Trypanosoma - classification
Trypanosoma - genetics
Trypanosoma - isolation & purification
TRYPANOSOMA BRUCEI
Trypanosoma brucei brucei - genetics
Trypanosoma brucei brucei - isolation & purification
TRYPANOSOMA CONGOLENSE
Trypanosoma congolense - genetics
Trypanosoma congolense - isolation & purification
Trypanosoma spp
TRYPANOSOMA VIVAX
Trypanosoma vivax - genetics
Trypanosoma vivax - isolation & purification
Trypanosomiasis, African - diagnosis
Trypanosomiasis, African - epidemiology
Trypanosomiasis, African - veterinary
Trypanosomiasis, Bovine - diagnosis
Trypanosomiasis, Bovine - epidemiology
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Summary:African animal trypanosomoses constitute the most important vector-borne cattle diseases in sub-Saharan Africa. Generally it is considered that there is a great lack of accurate tools for the diagnosis of the disease. During a trypanosomosis survey in the agro-pastoral zone of Sideradougou, Burkina Faso, 1036 cattle were examined for trypanosomes using microscopy. The PCR was applied on a subset of 260 buffy-coat samples using primers specific for Trypanosoma congolense savannah and riverine-forest groups, T. vivax, and T. brucei. Parasitological examination and the molecular technique were compared, showing a better efficiency of the latter. In the near future, the PCR is likely to become an efficient tool to estimate the prevalence of African trypanosomoses in affected areas.
ISSN:0304-4017
1873-2550
DOI:10.1016/S0304-4017(99)00137-5