Capillary electrophoresis with laser-induced fluorescence: a routine method to determine moxifloxacin in human body fluids in very small sample volumes

The feasibility of capillary electrophoresis with HeCd laser-induced fluorescence detection as a validated routine method for bioanalytical analysis is reported. Method evaluation, validation and results of the determination of moxifloxacin (BAY 12-8039), a new antimicrobially active 8-methoxy-quino...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1998-09, Vol.716 (1), p.325-334
Main Authors: Möller, J.-G, Staß, H, Heinig, R, Blaschke, G
Format: Article
Language:English
Subjects:
Analysis
Anti-Infective Agents - analysis
Anti-Infective Agents - blood
Aza Compounds
Biological and medical sciences
Body Fluids - chemistry
Chromatography, High Pressure Liquid
Electrophoresis, Capillary - methods
Fluorescence
Fluoroquinolones
General pharmacology
Humans
Lasers
Male
Medical sciences
Microchemistry
Microdialysate
Microdialysis
Moxifloxacin
Pharmacology. Drug treatments
Quality Control
Quinolines
Sensitivity and Specificity
Spectrophotometry
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Summary:The feasibility of capillary electrophoresis with HeCd laser-induced fluorescence detection as a validated routine method for bioanalytical analysis is reported. Method evaluation, validation and results of the determination of moxifloxacin (BAY 12-8039), a new antimicrobially active 8-methoxy-quinolone, in plasma and microdialysate are described. After a one step sample preparation the samples can be injected directly into the capillary. The volume of microdialysate and plasma, respectively, needed for more than 50 injections is only 10 μl and 20 μl. Total run time is less than 7 min using a 27 cm capillary on commercial instrumentation. An analysis time of less than 1 min was shown to be possible, however it could not be used routinely since appropriate instrumentation was not available. Evaluation is based on the relative corrected peak area (analyte/I.S.). The method's dynamic range comprises three orders of magnitude (plasma: 2.5–5000 μg/l; microdialysate: 5–5000 μg/l). Validation according to international guidelines yielded data on accuracy and precision of the method throughout the entire working range of inter-day precision: plasma
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(98)00302-8