Brush border enzyme activities in the small intestine after long-term gliadin feeding in animal models of human coeliac disease

Coeliac disease is a human, genetically linked, disorder which develops in gluten-sensitive persons. The aim of this study was to investigate the effect of prolonged feeding of gliadin, a major fraction of gluten, on enzyme activities of enterocyte brush border membrane enzymes in rats, mice and pig...

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Bibliographic Details
Published in:Folia microbiologica 1998-01, Vol.43 (5), p.497-500
Main Authors: Kozáková, H, Stĕpánková, R, Kolínská, J, Farré, M A, Funda, D P, Tucková, L, Tlaskalová-Hogenová, H
Format: Article
Language:English
Subjects:
Animal models
Animals
beta-Galactosidase - metabolism
Brushes
Celiac disease
Celiac Disease - enzymology
Dipeptidyl Peptidase 4 - metabolism
Dipeptidyl-peptidase IV
Disease Models, Animal
Enzymatic activity
Gliadin
Gliadin - administration & dosage
Gluten
Humans
Ileum
Intestinal Mucosa - enzymology
Intestine
Intestine, Small - enzymology
Jejunum
Juveniles
Lactase
Membranes
Mice
Mice, Inbred BALB C
Mice, Nude
Microvilli - enzymology
Rats
Rats, Wistar
Rodents
Small intestine
Sucrase - metabolism
Swine
Time Factors
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Summary:Coeliac disease is a human, genetically linked, disorder which develops in gluten-sensitive persons. The aim of this study was to investigate the effect of prolonged feeding of gliadin, a major fraction of gluten, on enzyme activities of enterocyte brush border membrane enzymes in rats, mice and pigs. Brush-border membranes were isolated from mucosal scrapings of the small intestine of 21-d-old rat pups hand-fed with formula milk diet, two-month-old nu/nu and +/+ BALB/c mice and two-month-old piglets fed three times a week starting at birth with high doses of gliadin. Activities of lactase, sucrase and dipeptidyl peptidase IV (DPP IV) were determined. Individual animal models differed in their response to gliadin feeding. In comparison with albumin fed controls the activities of DPP IV and lactase were decreased in rat pups, nu/nu BALB/c mice and piglets. DPP IV activity was mostly affected in the ileum of rats and piglets fed with gliadin starting at birth. On the other hand, lactase and sucrase activities of nu/nu BALB/c mice and piglets decreased to the largest extent in jejunum.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02820803