Convenient Transformation of Anamorphic Basidiomycetous Yeasts Belonging to Genus Pseudozyma Induced by Electroporation

A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/μg of plasmid DNA. Tran...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2007-12, Vol.104 (6), p.517-520
Main Authors: Morita, Tomotake, Habe, Hiroshi, Fukuoka, Tokuma, Imura, Tomohiro, Kitamoto, Dai
Format: Article
Language:English
Subjects:
Basidiomycota - classification
Basidiomycota - genetics
Biological and medical sciences
Biotechnology
DNA, Fungal - genetics
ELECTROPORACION
ELECTROPORATION
Electroporation - methods
EXPERIMENTACION
EXPERIMENTATION
Fundamental and applied biological sciences. Psychology
Gene Transfer Techniques
GENETIC TRANSFORMATION
green fluorescent protein (GFP)
hygromycin B
LEVADURA
LEVURE
PLASMIDE
PLASMIDIOS
PLASMIDS
Plasmids - genetics
Pseudozyma
Pseudozyma antarctica
TRANSFORMACION GENETICA
transformation
TRANSFORMATION GENETIQUE
Transformation, Genetic - genetics
yeast
YEASTS
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Summary:A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/μg of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure.
ISSN:1389-1723
1347-4421
DOI:10.1263/jbb.104.517