Advancements toward magneto immunoassays

Recently, a magneto binding assay was conducted. The operational principle is based on a `sandwich' mode of detection, where the target analyte (Concanavalin A) becomes bound nonselectively by protein adsorption between the solid support (silica carrier particles) and the ferromagnetic label (m...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1998-10, Vol.13 (7), p.817-823
Main Authors: Kriz, Kirstin, Gehrke, Janin, Kriz, Dario
Format: Article
Language:English
Subjects:
Animals
Ferrofluid
Ferromagnetic markers
Humans
Immunoassay - methods
Magnetics
Magneto immunoassay
MIA
Sensitivity and Specificity
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Summary:Recently, a magneto binding assay was conducted. The operational principle is based on a `sandwich' mode of detection, where the target analyte (Concanavalin A) becomes bound nonselectively by protein adsorption between the solid support (silica carrier particles) and the ferromagnetic label (magneto markers). A magnetic transducer was employed for the detection. The binding assay gave a linear response in the dynamic range of 0–1.9 μM and proved to be sensitive by having a limit of detection at 250 nM. Furthermore, the relative standard deviation observed was 6.2% ( n=3). The demonstration of the `sandwich' approach is a step towards achieving magneto immunoassays (MIA).
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(98)00047-5