The Alloreactive T Cell Response Against the Class Ib Molecule H2-M3 Is Specific for High Affinity Peptides

MHC class Ib molecule H2-M3 presents N-formylated peptides to CD8+ CTLs. Endogenous formylated peptides can come from the N-terminus of each of the 13 proteins encoded by the mitochondrial genome. In peptide competition assays, two of these peptides bind with high affinity, six bind with intermediat...

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Bibliographic Details
Published in:The Journal of immunology (1950) 1998-11, Vol.161 (10), p.5171-5178
Main Authors: Dabhi, Vikram M, Hovik, Rolf, Van Kaer, Luc, Fischer Lindahl, Kirsten
Format: Article
Language:English
Subjects:
Alleles
Animals
ATP-Binding Cassette Sub-Family B Member 2
ATP-Binding Cassette Transporters - immunology
ATP-Binding Cassette, Sub-Family B, Member 3
Cell Line
Chloramphenicol - pharmacology
Clone Cells
Cytotoxicity, Immunologic - drug effects
Epitopes, T-Lymphocyte - immunology
Epitopes, T-Lymphocyte - metabolism
Histocompatibility Antigens Class I - biosynthesis
Histocompatibility Antigens Class I - genetics
Histocompatibility Antigens Class I - immunology
Isoantigens - biosynthesis
Isoantigens - immunology
Lymphocyte Culture Test, Mixed
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred NZB
Mitochondria - enzymology
Mitochondria - immunology
Mitochondria - metabolism
N-Formylmethionine - metabolism
NADH Dehydrogenase - immunology
Oligopeptides - immunology
Oligopeptides - metabolism
Protein Binding - immunology
Protein Synthesis Inhibitors - pharmacology
T-Lymphocytes, Cytotoxic - immunology
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Summary:MHC class Ib molecule H2-M3 presents N-formylated peptides to CD8+ CTLs. Endogenous formylated peptides can come from the N-terminus of each of the 13 proteins encoded by the mitochondrial genome. In peptide competition assays, two of these peptides bind with high affinity, six bind with intermediate affinity, three bind with low affinity, and two do not bind measurably. Alloreactive CTLs from M3-specific, mixed lymphocyte cultures responded strongly against the two peptides with high affinity for M3, occasionally to peptides with intermediate affinity, and not at all to the rest. Long term lines and CTL clones reacted with only the high affinity peptides, demonstrating that alloreactive CTLs depend on specific peptides and that peptide affinity for class I correlates with alloantigenicity.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.161.10.5171