Comparison of two transportation media for study of normal individual conjunctival microbiota

To compare the microbiological profile of normal microbiota of healthy people obtained from conjunctival smear using dry swab in Stuart's transport medium and wet swab transported in test tube sealed with cotton. A prospective study with random samples, performed at the Departments of Ophthalmo...

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Bibliographic Details
Published in:Arquivos brasileiros de oftalmologia 2007-11, Vol.70 (6), p.929-934
Main Authors: Nogueira, Daniel Cruz, Ueda, Suely Mitoi Ykko, Murça, Maria Aparecida Soares, Hida, Wilson Takashi, Felberg, Sergio, Serruya, Leão, Hida, Richard Yudi
Format: Article
Language:Portuguese
Subjects:
Adult
Bacteriological Techniques - instrumentation
Bacteriological Techniques - methods
Colony Count, Microbial - methods
Conjunctiva - microbiology
Culture Media
Data Interpretation, Statistical
Female
Gram-Negative Bacteria - growth & development
Humans
Male
Prospective Studies
Staphylococcus - growth & development
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Summary:To compare the microbiological profile of normal microbiota of healthy people obtained from conjunctival smear using dry swab in Stuart's transport medium and wet swab transported in test tube sealed with cotton. A prospective study with random samples, performed at the Departments of Ophthalmology and Pathology of Santa Casa Misericórdia de São Paulo, in August of 2006. Eighty normal eyes of 40 healthy individuals were analyzed. Samples were collected in the right eye with a dry swab and stored in Stuart's medium, where all microbiological material is kept immersed in the medium and the tube is hermetically sealed. In the left eye, the conjunctival material was collected using a swab embedded in saline solution 0.9%, and stored in dry and sterile test tubes sealed with cotton. The samples were analyzed within 2 hours at most after collection. Out of 40 samples collected with wet swab and transported in dry tube, bacteria were observed in 10 (25%), whereas of 40 samples collected with dry swab and transported in Stuart's medium, 12 (30%) had bacteria. The results of the microbiological profile of normal conjunctival microbiota using dry swab in Stuart's medium were statistically similar (p=0.85) to those obtained in wet swab in dry tube for spreading performed within 2 hours after collection of conjunctival specimen.
ISSN:0004-2749