Spatial distribution of mineralized bone matrix produced by marrow mesenchymal stem cells in self-assembling peptide hydrogel scaffold

We evaluated the osteogenic differentiation of mesenchymal stem cells (MSCs) using a new class of synthetic self‐assembling peptide hydrogels, RADA 16, as a scaffold for three‐dimensional culture. MSCs derived from rat bone marrow were culture‐expanded and seeded into the hydrogel and further cultur...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2008-01, Vol.84A (1), p.128-136
Main Authors: Hamada, Kazunori, Hirose, Motohiro, Yamashita, Toshihiko, Ohgushi, Hajime
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Animals
Bone Density - drug effects
Bone Matrix - drug effects
Bone Matrix - metabolism
Cell Differentiation - drug effects
Cell Survival - drug effects
Cells, Cultured
Fluoresceins - metabolism
Hydrogels - chemistry
Male
mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - metabolism
mineralization
Osteocalcin - metabolism
osteogenic differentiation
Peptides - chemistry
Peptides - pharmacology
Rats
Rats, Inbred F344
RNA, Messenger - genetics
self-assembling peptide hydrogels
three-dimensional visualization
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Summary:We evaluated the osteogenic differentiation of mesenchymal stem cells (MSCs) using a new class of synthetic self‐assembling peptide hydrogels, RADA 16, as a scaffold for three‐dimensional culture. MSCs derived from rat bone marrow were culture‐expanded and seeded into the hydrogel and further cultured in osteogenic medium containing β‐glycerophosphate, ascorbic acid, and dexamethasone for 2–4 weeks. High alkaline phosphatase activity and osteocalcin (OC) contents were detected at both the protein and gene expression levels during the culture periods. Both calcium and the OC contents increased over time, indicating the growth of a mineralized extracellular matrix within the hydrogel. Moreover, the process of the growth of the mineralized matrix determined by three‐dimensional microarchitecture images was obtained by confocal laser scanning microscopy. The findings show that MSCs can differentiate into mature osteoblasts to form mineralized matrices within the hydrogel scaffold. Importantly, the differentiation can occur three dimensionally within the hydrogel, indicating that RADA 16 can be considered attractive synthetic biomaterial for use in bone tissue engineering. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.31439