Structure of a new neutral O-specific polysaccharide of Proteus penneri 34

The O-specific polysaccharide of Proteus penneri strain 34 was studied using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, and H-detected 1H, 13C HMQC experiments. The following structure was established, which is unique among the known structures of Proteus O-antigens: →4)- β- d-Glc...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate research 1998-11, Vol.312 (1), p.97-101
Main Authors: Toukach, Filip V, Arbatsky, Nikolay P, Shashkov, Alexander S, Knirel, Yuriy A, Zych, Krystyna, Sidorczyk, Zygmunt
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Carbohydrate Sequence
Lipopolysaccharide
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens - chemistry
O-antigen
O-specific polysaccharide
Proteus - chemistry
Proteus penneri
Rabbits
Structure
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The O-specific polysaccharide of Proteus penneri strain 34 was studied using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, and H-detected 1H, 13C HMQC experiments. The following structure was established, which is unique among the known structures of Proteus O-antigens: →4)- β- d-Glc p-(1⃗3)- β- d-Gal pNAc-(1⃗4)- β- d-Gal pNAc-(1⃗4)- β- d-Gal p-(1⃗. Accordingly, no cross-reaction was observed between P. penneri 34 O-antiserum and O-antigens of other Proteus strains. Therefore, the strain studied should belong to a new Proteus serogroup O65.
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(98)00232-8