Ischaemic preconditioning reduces infarct size following global ischaemia in the murine myocardium

Ischaemic preconditioning has not been demonstrated to reduce infarct size following global ischaemia in murine myocardium. Eighteen mouse hearts were isolated, perfused in the Langendorff mode, and randomised to control or preconditioning groups. Preconditioned hearts received four periods of five...

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Bibliographic Details
Published in:Basic research in cardiology 1998-10, Vol.93 (5), p.384-390
Main Authors: Sumeray, M S, Yellon, D M
Format: Article
Language:English
Subjects:
Animals
Arrhythmias, Cardiac - pathology
Arrhythmias, Cardiac - physiopathology
Arrhythmias, Cardiac - therapy
Coronary Circulation - physiology
Ischemic Preconditioning, Myocardial
L-Lactate Dehydrogenase - metabolism
Male
Mice
Muscle Contraction - physiology
Muscle Fibers, Skeletal - physiology
Myocardial Infarction - pathology
Myocardial Infarction - physiopathology
Myocardial Infarction - therapy
Myocardial Ischemia - pathology
Myocardial Ischemia - physiopathology
Myocardial Ischemia - therapy
Myocardium - enzymology
Myocardium - pathology
Reperfusion Injury - pathology
Reperfusion Injury - physiopathology
Reperfusion Injury - therapy
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Summary:Ischaemic preconditioning has not been demonstrated to reduce infarct size following global ischaemia in murine myocardium. Eighteen mouse hearts were isolated, perfused in the Langendorff mode, and randomised to control or preconditioning groups. Preconditioned hearts received four periods of five minutes global ischaemia with five minutes of intervening reperfusion. Control hearts were perfused normally during this period. Both groups were then subjected to 30 minutes global ischaemia followed by 30 minutes reperfusion. Infarct size, contractile recovery and LDH leakage were assessed. Mean infarct size was reduced from 57% of ventricular volume (controls) to 33% in preconditioned hearts (P = 0.003). A small improvement in contractile recovery was also observed (6.3% of baseline in controls, 15.5% in preconditioned hearts; P = 0.004). Release of LDH did not differ significantly between groups. This study confirms for the first time that ischaemic preconditioning can delay the onset of myocardial necrosis following global ischaemia in the isolated mouse heart.
ISSN:0300-8428
1435-1803
DOI:10.1007/s003950050106