Identification and real-time imaging of a myc-expressing neutrophil population involved in inflammation and mycobacterial granuloma formation in zebrafish

By enhancer trap screening we identified a transgenic zebrafish line showing leukocyte-specific YFP expression during late embryo and early larval development. Its enhancer detection insertion was mapped near a novel member of the myc proto-oncogene family, encoding transcription factors known to be...

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Bibliographic Details
Published in:Developmental and comparative immunology 2008, Vol.32 (1), p.36-49
Main Authors: Meijer, Annemarie H., van der Sar, Astrid M., Cunha, Cristiana, Lamers, Gerda E.M., Laplante, Mary A., Kikuta, Hiroshi, Bitter, Wilbert, Becker, Thomas S., Spaink, Herman P.
Format: Article
Language:English
Subjects:
Animals
Cell Movement
Danio rerio
Embryo, Nonmammalian
Freshwater
Granuloma - immunology
Granuloma - microbiology
Infectious disease
Inflammation
Inflammation - immunology
Inflammation - metabolism
Myc genes
Mycobacterium
Mycobacterium Infections, Nontuberculous - immunology
Mycobacterium Infections, Nontuberculous - microbiology
Mycobacterium marinum - physiology
Myeloid development
Neutrophils
Neutrophils - immunology
Neutrophils - metabolism
Phylogeny
Proto-Oncogene Proteins c-myc - biosynthesis
Transgenic model
Zebrafish
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Summary:By enhancer trap screening we identified a transgenic zebrafish line showing leukocyte-specific YFP expression during late embryo and early larval development. Its enhancer detection insertion was mapped near a novel member of the myc proto-oncogene family, encoding transcription factors known to be important for regulating human myelopoiesis. Characterization of the zebrafish myc family showed that only this particular myc gene is strongly expressed in leukocytes. To identify the myc/YFP-expressing cell type, we re-examined specificity of described myeloid markers by multiplex fluorescent in situ hybridization, showing that lcp1 can be considered as a general leukocyte marker, csf1r as a macrophage-specific marker, and mpx and lyz as neutrophil-specific markers. Subsequent colocalization analysis defined the YFP-positive cells as a subset of the neutrophil population. Using real-time confocal imaging we demonstrate that these cells migrate to sites of inflammation and are involved in innate immune responses towards infections, including Mycobacterium marinum-induced granuloma formation.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2007.04.003