Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations

To study the ganglion cells (GC) in the terminal bowel of rats with ethylenethiourea (ETU) induced anorectal malformations (ARM). The animals were divided into three groups: Group A--normal fetuses from pregnant rats that were not administered ETU; Group B--fetuses without ARM born from pregnant rat...

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Bibliographic Details
Published in:Acta cirúrgica brasileira 2007-11, Vol.22 (6), p.441-445
Main Authors: Macedo, Maurício, Martins, José Luiz, Meyer, Karine Furtado, Soares, Iberê Cauduro
Format: Article
Language:English
Subjects:
Anal Canal - abnormalities
Anal Canal - innervation
Anal Canal - pathology
Animals
Cell Count
Disease Models, Animal
Enteric Nervous System - drug effects
Enteric Nervous System - pathology
Ethylenethiourea
Female
Ganglia - cytology
Immunohistochemistry
Pregnancy
Rats
Rats, Wistar
Rectum - abnormalities
Rectum - innervation
Rectum - pathology
S100 Proteins - analysis
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Summary:To study the ganglion cells (GC) in the terminal bowel of rats with ethylenethiourea (ETU) induced anorectal malformations (ARM). The animals were divided into three groups: Group A--normal fetuses from pregnant rats that were not administered ETU; Group B--fetuses without ARM born from pregnant rats that were administered ETU and Group C--fetuses with ARM born from pregnant rats that received ETU. ETU was administered on the 11th day of pregnancy at the dose of 125 mg/kg body weight by gastric gavage. The rats had cesarean section on the 21st day of gestation. The fetuses terminal bowel tissue was analyzed by immunohistochemistry to demonstrate ganglion cells. Statistically significant differences were found between groups A, B and C regarding ganglion cell densities. Group A had the highest cell density, followed by Group B and the lowest density was found in Group C. Ganglion cell densities are decreased in the terminal bowel of rats with ARM.
ISSN:0102-8650
0102-8650
DOI:10.1590/S0102-86502007000600005