Role of superoxide anion on the proliferation and c-Ha-ras or p53 expression in prostate cancer cell line PC3

• Published in: Urolithiasis 1998-10, Vol.26 (5), p.349-353
• Main Authors: Wang, Z, Zheng, R, Fu, S, Chen, Y, Duan, G, Qin, D, Liu, G
• Format: Article
• Language: English
• Subjects: Anions - metabolism; Fibroblast Growth Factor 2 - pharmacology; Gene expression; Gene Expression Regulation, Neoplastic - drug effects; Gene Expression Regulation, Neoplastic - physiology; Humans; Male; Oncogene Protein p21(ras) - genetics; Prostate cancer; Prostatic Neoplasms; Superoxides - metabolism; Superoxides - pharmacology; Tumor Cells, Cultured - drug effects; Tumor Cells, Cultured - physiology; Tumor Suppressor Protein p53 - genetics
• ISSN: 0300-5623; 2194-7228; 1434-0879; 2194-7236
• DOI: 10.1007/s002400050068

The purpose of this study was to investigate the role of superoxide anion (02-*) in the regulation of p53 or c-Ha-ras expression and proliferation in the prostate cancer cell line PC3. Cell proliferation was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in the presence of O2-*, basic fibroblast growth factor (bFGF) or their combination. p53 or C-Ha-ras expression in the cells treated with O2-* was assayed by fluorescence in situ hybridization (FISH). The proliferation was significantly inhibited by O2-* in a concentration-dependent manner ranging from 9 to 36 micromol/l nicotinamide adenine dinucleotid (NADH) combined with 2-8 micromol/l N-methylphenazonium methyl sulfate (PMS). Enhancement of proliferation by 2 ng/ml bFGF was significantly inhibited by O2-*. Although O2-* was not able to alter c-Ha-ras gene expression, O2-* at the concentrations of 18 micromol/l NADH and 4 micromol/l PMS upregulated the expression of p53. O2-* may modulate proliferation and gene expression in PC3 cells.