predictive nutritional model for plant cells and hairy roots

• Published in: Biotechnology and bioengineering 2008, Vol.99 (1), p.189-200
• Main Authors: Cloutier, M, Bouchard-Marchand, É, Perrier, M, Jolicoeur, M
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; Botany; Carbohydrate Metabolism - physiology; Catharanthus roseus; Cell culture; Cell growth; cell nutrition; Cell Physiological Phenomena; Cell Proliferation; Computer Simulation; Daucus carota; Eukaryotic cell cultures; Flowers & plants; Fundamental and applied biological sciences. Psychology; hairy roots; kinetic model; Kinetics; Methods. Procedures. Technologies; Miscellaneous; modeling; Models, Biological; Nitrogen Compounds - metabolism; Nutrition; Phosphates - metabolism; plant cells; Plant cells and fungal cells; Plant Physiological Phenomena; Plant Roots - physiology
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.21543

A structured nutritional model is proposed to describe growth and nutritional behavior of Eschscholtzia californica suspension cells and Catharanthus roseus and Daucus carota hairy roots in in vitro culture. The model describes the cells specific growth rate from concentration of intracellular nutrients such as inorganic phosphate (Pi), nitrogen sources (NO₃⁻ and NH₄⁺) and sugars. Two-level Michaelis-Menten kinetics are used to describe Pi and NO₃⁻ uptake and simple Michaelis-Menten kinetics for description of sugars uptake. Model parameters for each cell line were calibrated using data from batch cultures. The predictive capacity of the model was tested using data from medium exchange hairy root cultures. The model describes growth and nutritional behavior for the cell and hairy root lines. A sensitivity analysis was performed to identify critical model parameters and effect of initial conditions. The cell and hairy roots lines are also compared from their kinetic parameters. The kinetic model is efficient for describing and predicting growth and nutritional behaviors of suspension cells and hairy roots. Biotechnol. Bioeng. 2008;99: 189-200. © 2007 Wiley Periodicals, Inc.