Cytokines and chemokines in postovulatory follicle regression of domestic chicken ( Gallus gallus domesticus)

• Published in: Developmental and comparative immunology 2008, Vol.32 (3), p.253-264
• Main Authors: Sundaresan, N.R., Saxena, V.K., Sastry, K.V.H., Nagarajan, K., Jain, Preeti, Singh, Rani, Anish, D., Ravindra, P.V., Saxena, M., Ahmed, K.A.
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Apoptosis - genetics; Apoptosis - physiology; Chemokines; Chemokines - genetics; Chicken; Chickens - genetics; Chickens - physiology; Cytokines; Cytokines - genetics; Female; Gallus gallus domesticus; Gene Expression; Immune cells; Interferon-gamma - genetics; Interleukins - genetics; Lymphocyte Subsets - cytology; Lymphocyte Subsets - metabolism; Ovarian Follicle - cytology; Ovarian Follicle - metabolism; Ovary - cytology; Ovary - metabolism; Ovulation - genetics; Ovulation - physiology; Postovulatory follicle; Reverse Transcriptase Polymerase Chain Reaction; Transforming Growth Factor beta2 - genetics
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2007.05.011

The mechanism of postovulatory follicle (POF) regression in birds is still poorly understood. In the current study, expression of IL-1 β, IL-6, GM-CSF, IFN- γ, IL-2, IL-4, IL-13, chCXCLi2, chCCLi2, chCCLi4, chCCLi7, IL-10 and TGF- β2 mRNAs was estimated in regressing POF by semi-quantitative RT-PCR. In addition, the changes in immune cell population, histological and apoptotic changes were also studied in regressing POF. The expression of cytokines (IL-1 β, IL-6, IL-10 and TGF- β2) and chemokines (chCXCLi2, chCCLi2, chCCLi4 and chCCLi7) was upregulated in POFs, suggesting a role for these molecules in tissue regression. The histological findings suggested a significant infiltration of immune cells, especially heterophils, lymphocytes and macrophages, into the regressing POF. The flow cytometry analysis of lymphocyte subpopulations revealed that CD3 +, CD4 +, CD8 + and Bu-1 + lymphocytes were significantly increased during this regression. The significant up-regulation of chemokines might have attracted the immune cells during POF regression. The percentage of apoptotic cells was significantly increased during the regression of POF. The up-regulation of IL-1 β, IL-6, IL-10 and TGF- β2 and down-regulation of GM-CSF might have induced apoptosis during the POF regression. However, expression of IFN- γ, IL-2, IL-4 and IL-13 was not significantly altered during POF regression. In conclusion, cytokines appear to play an important role in the regression of POF in chicken. Furthermore, the regression of chicken POF seems to be an inflammatory event similar to luteolysis of the mammalian corpus luteum.