Rat Primary Hepatocytes and H4 Hepatoma Cells Display Differential Sensitivity to Cyclic AMP at the Level of Expression of Tyrosine Aminotransferase

We have shown that the sensitivity of isolated hepatocytes and H4 hepatoma cells to cyclic AMP is different. In terms of activation of tyrosine aminotransferase at mRNA and activity level in response to cyclic AMP, isolated hepatocytes are 10-fold more sensitive. Hepatocytes and H4 hepatoma cells sh...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1998-11, Vol.252 (3), p.764-769
Main Authors: Pickering, Clive S., Watkins, Rachel H., Dickson, Alan J.
Format: Article
Language:English
Subjects:
Animals
CREB
Cyclic AMP - pharmacology
Cyclic AMP Response Element-Binding Protein - metabolism
Cyclic AMP-Dependent Protein Kinases - metabolism
Enzyme Induction
Liver - drug effects
Liver - enzymology
Liver Neoplasms, Experimental - enzymology
Male
protein kinase A
Rats
Rats, Sprague-Dawley
signaling
transcriptional control
Tumor Cells, Cultured
tyrosine aminotransferase
Tyrosine Transaminase - biosynthesis
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Summary:We have shown that the sensitivity of isolated hepatocytes and H4 hepatoma cells to cyclic AMP is different. In terms of activation of tyrosine aminotransferase at mRNA and activity level in response to cyclic AMP, isolated hepatocytes are 10-fold more sensitive. Hepatocytes and H4 hepatoma cells show similar sensitivities to cyclic AMP at the level of protein kinase A activation and phosphorylation of cyclic AMP response element binding protein (CREB) and the differential sensitivity must reside at other sites. The consequences of these findings to studies of control phenomena at the transcriptional level is discussed.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.9735