Adhesion and viability of waterborne pathogens on p-DADMAC coatings

The attachment of waterborne pathogens onto surfaces can be increased by coating the surfaces with positive charge-enhancing polymers. In this paper, the increased efficacy of polydiallyldimethylammonium chloride (p-DADMAC) coatings on glass was evaluated in a parallel plate flow chamber with the us...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and bioengineering 2008, Vol.99 (1), p.165-169
Main Authors: van der Mei, Henny C, Rustema-Abbing, Minie, Langworthy, Don E, Collias, Dimitris I, Mitchell, Michael D, Bjorkquist, Dave W, Busscher, Henk J
Format: Article
Language:English
Subjects:
Allyl Compounds - chemistry
Aqueous solutions
Bacterial Adhesion - physiology
Biological and medical sciences
Brevundimonas diminuta
Cell adhesion & migration
Cell Survival
Coated Materials, Biocompatible - chemistry
E coli
Escherichia coli
Fundamental and applied biological sciences. Psychology
Materials Testing
Microbiology
Pathogens
polydiallyldimethylammonium chloride
Quaternary Ammonium Compounds - chemistry
R. terrigena
Raoultella terrigena
Studies
Surface Properties
Water Microbiology
Water treatment
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The attachment of waterborne pathogens onto surfaces can be increased by coating the surfaces with positive charge-enhancing polymers. In this paper, the increased efficacy of polydiallyldimethylammonium chloride (p-DADMAC) coatings on glass was evaluated in a parallel plate flow chamber with the use of waterborne pathogens (Raoultella terrigena, Escherichia coli, and Brevundimonas diminuta). p-DADMAC coatings strongly compensated the highly negative charges on the glass surface and even yielded a positively charged surface when applied from a 500 ppm solution. Whereas none of the strains adhered from water to glass due to electrostatic repulsion, R. terrigena and E. coli readily adhered in high numbers to p-DADMAC coated glass slides applied from 1, 100, or 500 ppm aqueous solutions. B. diminuta only adhered to a positively charged p-DADMAC coating applied from a 500 ppm solution. In addition, all p-DADMAC coatings indicated strong contact killing with the bacterial species used in this study by live/dead staining techniques. In summary, this paper demonstrates the potential of p-DADMAC coatings to strongly enhance bacterial adhesion. Moreover, once adhered, bacterial viability can be reduced by the positively charged ammonium groups in the coating. Biotechnol. Bioeng. 2008;99: 165-169. © 2007 Wiley Periodicals, Inc.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.21538