Automated RNA Selection

In vitro selection can be used to generate nucleic acid ligands (aptamers) to target molecules ranging in size and structure from cations to cells. However, the selection process is repetitive and time‐consuming. We have automated a protocol for in vitro selection using an augmented Beckman Biomek 2...

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Bibliographic Details
Published in:Biotechnology progress 1998-11, Vol.14 (6), p.845-850
Main Authors: Cox, J. Colin, Rudolph, Peter, Ellington, Andrew D.
Format: Article
Language:English
Subjects:
Base Composition
Base Sequence
Biological and medical sciences
Biotechnology
Biotechnology - instrumentation
Biotechnology - methods
Cells
Diverse techniques
DNA Primers
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Molecular and cellular biology
Molecular Biology - instrumentation
Molecular Biology - methods
Molecular Sequence Data
Molecular structure
Others
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA - chemistry
RNA - genetics
Robot applications
Robotics
Software
Transcription, Genetic
Various methods and equipments
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Description
Summary:In vitro selection can be used to generate nucleic acid ligands (aptamers) to target molecules ranging in size and structure from cations to cells. However, the selection process is repetitive and time‐consuming. We have automated a protocol for in vitro selection using an augmented Beckman Biomek 2000 pipetting robot. The automated selection procedure requires the integration of four devices and the optimization of four molecular biology methods, and is one of the most complex automated protocols attempted to date. Initial attempts at selection yielded robust replication parasites, but optimization of the automated selection procedure suppressed the emergence of these parasites and led to the selection of true nucleic acid ligands. Automated selection can now be used to generate nucleic acid aptamers in days rather than weeks or months.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp980097h