RA70 Is a Src Kinase-Associated Protein Expressed Ubiquitously

RA70,which is expressed during neuronal differentiation of P19 EC, is highly homologous to human src kinase-associated phosphoprotein (SKAP55). Here we isolated human full-lengthRA70cDNA. UnlikeSKAP55,which is specifically expressed in thymus and T cells,RA70was expressed ubiquitously in various tis...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1998-11, Vol.252 (3), p.738-742
Main Authors: Kouroku, Yoriko, Soyama, Akiko, Fujita, Eriko, Urase, Koko, Tsukahara, Toshifumi, Momoi, Takashi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Blood Proteins - chemistry
Cell Differentiation
Cell Line
Humans
Intracellular Signaling Peptides and Proteins
Jurkat Cells
Macrophages - cytology
Mice
Molecular Sequence Data
Monocytes - cytology
Phosphoproteins - chemistry
Phosphoproteins - metabolism
Phosphorylation
Polymerase Chain Reaction
Protein-Tyrosine Kinases - metabolism
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-fyn
Proto-Oncogene Proteins c-hck
Sequence Homology, Amino Acid
src Homology Domains
Tumor Cells, Cultured
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Summary:RA70,which is expressed during neuronal differentiation of P19 EC, is highly homologous to human src kinase-associated phosphoprotein (SKAP55). Here we isolated human full-lengthRA70cDNA. UnlikeSKAP55,which is specifically expressed in thymus and T cells,RA70was expressed ubiquitously in various tissues including lung, skeletal muscle, and spleen, and in various cell lines including human monocytic leukemia (U937) cells, butRA70was undetectable in thymus and T cell lymphoma (Jurkat) cells. RA70 as well as SKAP55 proved to be a protein with molecular weight 55 kDa associated with SH2 domain of Fyn. Interaction between RA70 and src family kinases, Fyn, Hck and Lyn, was detected during monocytes/macrophage-differentiation of U937 cells. Thus, like SKAP55, RA70 is an adaptor protein of the src family kinases. RA70 may play an essential role in the src signaling pathway in various cells.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.9637