Accelerated viraemia in cats vaccinated with fixed autologous FIV-infected cells

We have vaccinated cats with fixed autologous FIV infected PBMC to determine whether autologous presentation of antigen is capable of inducing a protective immune response against homologous challenge. To this end autologous PBMC were infected with a FIV molecular clone (19k1). When infection was es...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 1998-10, Vol.65 (2), p.353-365
Main Authors: Karlas, Jos A, Siebelink, Kees H.J, v. Peer, Maartje A, Huisman, Willem, Rimmelzwaan, Guus F, D.M.E. Osterhaus, Albert
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
Antibodies, Viral - analysis
Autologous vaccination
Blood Component Transfusion
Blood Transfusion, Autologous
Cats
DNA Primers - chemistry
DNA, Viral - analysis
Enhancement of infection
Feline Acquired Immunodeficiency Syndrome - immunology
Feline Acquired Immunodeficiency Syndrome - prevention & control
Feline immunodeficiency virus
FIV
Immunodeficiency Virus, Feline - genetics
Immunodeficiency Virus, Feline - immunology
Polymerase Chain Reaction - veterinary
T-Lymphocytes - immunology
T-Lymphocytes - virology
Vaccination - veterinary
Vaccines
Viral Load
Viral Vaccines - administration & dosage
Viremia - etiology
Viremia - immunology
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Summary:We have vaccinated cats with fixed autologous FIV infected PBMC to determine whether autologous presentation of antigen is capable of inducing a protective immune response against homologous challenge. To this end autologous PBMC were infected with a FIV molecular clone (19k1). When infection was established, cells were inactivated by dialysis against paraformaldehyde. Upon vaccination, cats developed a virus specific immune response as measured by ELISA against the Gag protein of FIV. No antibodies against the envelope protein were detected with a peptide ELISA. Virus neutralizing antibodies however could be detected with a neutralization assay based on infection of CrFK cells, but not in an assay based on infection of primary T-cells. Although vaccination led to the induction of these virus-specific immune responses, vaccinated cats were not protected against homologous challenge but showed an accelerated viraemia upon infection. This was shown both by PCR and cell-associated viral load. The possible mechanisms underlying this observation are discussed in this paper.
ISSN:0165-2427
1873-2534
DOI:10.1016/S0165-2427(98)00166-4