Ferric iron reduction by Desulfovibrio vulgaris Hildenborough wild type and energy metabolism mutants

Desulfovibrio vulgaris Hildenborough wild type and its hyn1, hyd and hmc mutants, lacking genes for periplasmic [NiFe] hydrogenase-1, periplasmic [FeFe] hydrogenase or the transmembrane high molecular weight cytochrome (Hmc) complex, respectively, were able to reduce Fe(III) chelated with nitrilotri...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2008-02, Vol.93 (1-2), p.79-85
Main Authors: Park, Hyung Soo, Lin, Shiping, Voordouw, Gerrit
Format: Article
Language:English
Subjects:
Anthraquinones - metabolism
Bacteria
Bacteriology
Biological and medical sciences
Biomedical and Life Sciences
Desulfovibrio vulgaris
Desulfovibrio vulgaris - genetics
Desulfovibrio vulgaris - metabolism
Energy Metabolism - genetics
Enzymes
Ferric Compounds - metabolism
Fundamental and applied biological sciences. Psychology
Genotype
Hydrogenase - metabolism
Iron
Iron - metabolism
Life Sciences
Marine
Medical Microbiology
Metabolism
Metabolism. Enzymes
Microbiology
Mutation
Nitrilotriacetic acid
Original Paper
Oxidation-Reduction
Plant Sciences
Soil Science & Conservation
Sulfate reduction
Sulfates
Sulfates - metabolism
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Summary:Desulfovibrio vulgaris Hildenborough wild type and its hyn1, hyd and hmc mutants, lacking genes for periplasmic [NiFe] hydrogenase-1, periplasmic [FeFe] hydrogenase or the transmembrane high molecular weight cytochrome (Hmc) complex, respectively, were able to reduce Fe(III) chelated with nitrilotriacetic acid (NTA), but not insoluble ferric oxide, with lactate as the electron donor. The rate and extent of Fe(III)-NTA reduction followed the order hyn = WT > hmc >> hyd, suggesting that reduction of soluble Fe(III) is a periplasmic process that requires the presence of periplasmic [FeFe] hydrogenase. Reduction of Fe(III)-NTA was not coupled to cell growth. In fact cell concentrations declined when D. vulgaris was incubated with Fe(III)-NTA as the only electron acceptor. Wild type and mutant cells reducing a limiting concentration of sulfate (2 mM), reduced Fe(III)-NTA with similar rates. However, these were similarly incapable of catalyzing subsequent lactate-dependent reduction of Fe(III)-NTA to completion. Periplasmic reduction of Fe(III)-NTA appeared to inhibit the productive, sulfate-reducing metabolism of D. vulgaris, possibly because it prevents the cycling of reducing equivalents needed to achieve a net bioenergetic benefit.
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-007-9181-3