Phosphoantigen activation induces surface translocation of intracellular CD94/NKG2A class I receptor on CD94- peripheral Vgamma9 Vdelta2 T cells but not on CD94- thymic or mature gammadelta T cell clones

Most adult peripheral blood gammadelta T cells express Vgamma9/Vdelta2-encoded TCR that recognize a restricted set of nonpeptidic phosphorylated compounds, referred to as phosphoantigens. They also express various MHC class I-specific inhibitory receptors (IR), in particular CD94/ NKG2-A heterodimer...

Full description

Saved in:
Bibliographic Details
Published in:European journal of immunology 1998-11, Vol.28 (11), p.3399-3410
Main Authors: Boullier, S, Poquet, Y, Halary, F, Bonneville, M, Fournie, J J, Gougeon, M L
Format: Article
Language:English
Subjects:
Animals
Antigens - immunology
Antigens, CD - analysis
Antigens, CD - metabolism
Biological Transport
Humans
Lectins, C-Type
Lymphocyte Activation
Membrane Glycoproteins - analysis
Membrane Glycoproteins - metabolism
Mice
NK Cell Lectin-Like Receptor Subfamily D
Receptors, Antigen, T-Cell, gamma-delta - analysis
T-Lymphocytes - immunology
Thymus Gland - cytology
Tumor Necrosis Factor-alpha - biosynthesis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Most adult peripheral blood gammadelta T cells express Vgamma9/Vdelta2-encoded TCR that recognize a restricted set of nonpeptidic phosphorylated compounds, referred to as phosphoantigens. They also express various MHC class I-specific inhibitory receptors (IR), in particular CD94/ NKG2-A heterodimers, which participate in the fine tuning of their TCR-mediated activation threshold. Most mature Vgamma9/Vdelta2 T cells express surface CD94 receptors, unlike cord blood or thymus-derived Vgamma9/Vdelta2 clones, thus suggesting a role for the microenvironment in IR expression. In the present study we show that most CD94- Vgamma9Vdelta2 PBL ex vivo express an intracellular pool of CD94/NKG2-A receptors that is translocated to the cell surface upon activation by phosphoantigens or IL-2. In stark contrast, intracellular CD94/NKG2-A complexes are undetectable in CD94- thymus or PBL-derived mature Vdelta2 T cell clones, and no surface induction is observed following phosphoantigen activation of T cell clones. Altogether these results provide new insights into the regulation of CD94/NKG2-A expression on T lymphocytes and suggest the existence of distinct mechanisms controlling in vivo and in vitro induction of IR on these cells.
ISSN:0014-2980