The effect of ovarian steroids on epithelial ciliary beat frequency in the human Fallopian tube

• Published in: Human reproduction (Oxford) 1998-11, Vol.13 (11), p.2991-2994
• Main Authors: Mahmood, T, Saridogan, E, Smutna, S, Habib, A M, Djahanbakhch, O
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Cilia - drug effects; Cilia - physiology; Epithelium - ultrastructure; Estradiol - pharmacology; Fallopian Tubes - ultrastructure; Female; Fundamental and applied biological sciences. Psychology; Hormone Antagonists - pharmacology; Hormone metabolism and regulation; Humans; Mammalian female genital system; Mifepristone - pharmacology; Progesterone - antagonists & inhibitors; Progesterone - pharmacology; Vertebrates: reproduction
• ISSN: 0268-1161; 1460-2350
• DOI: 10.1093/humrep/13.11.2991

Using a method that detects variations in light intensity we have studied the effect of ovarian steroids on human Fallopian tube epithelial ciliary beat frequency in vitro. We have found that baseline ciliary beat frequency averages between 5-6 Hz. Cilia from ampullary segments of the Fallopian tube beat significantly faster (5.4 Hz+/-0.2) than those from fimbrial segments (4.8 Hz+/-0.2). There was no significant difference in baseline ciliary beat frequency at any other anatomical site in the Fallopian tube. Incubation with progesterone (10 micromol/l) suppresses human Fallopian tube epithelial ciliary beat frequency by 40-50%. This inhibition was observed at similar magnitudes in all Fallopian tubes studied irrespective of anatomical site. Progesterone-induced reductions in ciliary beat frequency were concentration dependent and prevented by the progesterone receptor antagonist mifepristone (RU486). Oestradiol alone (10 micromol/l) had no effect on ciliary beat frequency at any anatomical site in the Fallopian tube but did prevent the reduction in ciliary beat frequency seen with progesterone when tissues were incubated with these two steroids together.