Binding of bivalent cations by hyaluronate in aqueous solution

The interaction between sodium hyaluronate and bivalent cations was investigated by conductometry, viscosimetry, circular dichroism and nuclear magnetic resonance spectroscopy. It is shown that the hyaluronate chains ( M n ∼4.0 × 10 5–1.7 × 10 6 g/mol) bind various bivalent cations (Ca 2+, Mg 2+, Mn...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2008, Vol.42 (1), p.33-40
Main Authors: Furth, Guido, Knierim, Robert, Buss, Volker, Mayer, Christian
Format: Article
Language:English
Subjects:
Binding
Cations
Cations, Divalent - chemistry
Circular Dichroism
Complex
Conductometry
Hyaluronate
Hyaluronic acid
Hyaluronic Acid - chemistry
Hyaluronic Acid - isolation & purification
Leuconostoc mesenteroides
Magnetic Resonance Spectroscopy
Metals - chemistry
Molecular Weight
Solutions
Streptococcus equi
Viscosity
Water - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The interaction between sodium hyaluronate and bivalent cations was investigated by conductometry, viscosimetry, circular dichroism and nuclear magnetic resonance spectroscopy. It is shown that the hyaluronate chains ( M n ∼4.0 × 10 5–1.7 × 10 6 g/mol) bind various bivalent cations (Ca 2+, Mg 2+, Mn 2+, Fe 2+, Cu 2+, Zn 2+, Cd 2+ and Pb 2+) at pH 6 in aqueous solutions. Hyaluronate deriving from Streptococcus equi was studied in comparison with dextran from Leuconostoc mesenteroides which was shown to develop no specific interactions with the bivalent cations. The molar relation between the bivalent cations and the disaccharide units of the resulting complex was determined with the result that one bivalent cation is bound by approximately five disaccharide units. Heavy metal ions (Cd 2+, Pb 2+) seem to bind stronger to the hyaluronate chain than their lighter counterparts (Ca 2+, Mg 2+). Circular dichroism spectra of the hyaluronate exhibit a cation-induced change in the n-π* transition, indicating that the acetamide group of the aminoglucane unit is involved during the complexation. NMR spectra of hyaluronic acid in presence of paramagnetic manganese cations show strong interactions between the acetamide as well as the carboxylate groups and the cations. Based on these data, a structure of the binding complex is proposed which involves two disaccharide units.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2007.09.001