Human interleukin 10 suppresses production of inflammatory mediators by LPS-stimulated equine peritoneal macrophages

To investigate the ability of recombinant human interleukin 10 (rhuIL-10) to suppress the release of inflammatory mediators from lipopolysaccharide (LPS)-stimulated equine macrophages, rhuIL-10 was added to equine peritoneal macrophage monolayers at concentrations of 0, 0.1, 1, 10, or 100 ng/ml. Thi...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 1998-11, Vol.66 (1), p.1-10
Main Authors: Hawkins, D.L, MacKay, R.J, MacKay, S.L.D, Moldawer, L.L
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Dinoprostone - biosynthesis
Equine peritoneal macrophages
Horses - immunology
Horses - metabolism
Humans
IL-10
IL-6
IMMUNODEPRESSEUR
IMMUNOSUPPRESSANTS
INMUNODEPRESORES
Interleukin-10 - pharmacology
Interleukin-6 - biosynthesis
INTERLEUKINE
INTERLEUKINS
INTERLEUQUINAS
LIPOPOLISACARIDOS
LIPOPOLYSACCHARIDE
LIPOPOLYSACCHARIDES
Lipopolysaccharides - pharmacology
LPS
MACROFAGOS
MACROPHAGE
MACROPHAGES
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - immunology
Macrophages, Peritoneal - metabolism
Nitric oxide
Nitric Oxide - biosynthesis
PGE 2
Recombinant Proteins - pharmacology
TNF
Tumor Necrosis Factor-alpha - biosynthesis
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Summary:To investigate the ability of recombinant human interleukin 10 (rhuIL-10) to suppress the release of inflammatory mediators from lipopolysaccharide (LPS)-stimulated equine macrophages, rhuIL-10 was added to equine peritoneal macrophage monolayers at concentrations of 0, 0.1, 1, 10, or 100 ng/ml. Thirty minutes later, LPS ( E. coli 055 : B5) was added at final concentrations of 0, 1, 10, 100 ng/ml. Macrophages were incubated for 16 h at 37°C, then supernates were harvested and assayed for tumor necrosis factor (TNF) activity (L929 cytotoxicity), interleukin-6 (IL-6) activity (B9 proliferation), prostaglandin E 2 concentration (ELISA), and nitric oxide (Griess reaction for nitrite). Preincubation of LPS-stimulated peritoneal macrophages with rhuIL-10 caused significant ( P
ISSN:0165-2427
1873-2534
DOI:10.1016/S0165-2427(98)00181-0