Acquired increase in leucocyte binding by intestinal microvascular endothelium in inflammatory bowel disease

• Published in: The Lancet (British edition) 1998-11, Vol.352 (9142), p.1742-1746
• Main Authors: Binion, David G, West, Gail A, Volk, Emily E, Drazba, Judith A, Ziats, Nicholas P, Petras, Robert E, Fiocchi, Claudio
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Cell Adhesion Molecules - analysis; Cells, Cultured; Colitis, Ulcerative - immunology; Colitis, Ulcerative - pathology; Crohn Disease - immunology; Crohn Disease - pathology; Digestive system; Endothelium, Vascular - immunology; Endothelium, Vascular - pathology; Female; Gastroenterology. Liver. Pancreas. Abdomen; Humans; In Vitro Techniques; Inflammatory bowel disease; Intestinal Mucosa - blood supply; Intestinal Mucosa - immunology; Intestinal Mucosa - pathology; Leukocyte Adherence Inhibition Test; Leukocytes; Leukocytes - immunology; Leukocytes - pathology; Male; Medical disorders; Medical sciences; Microcirculation - immunology; Microcirculation - pathology; Neutrophils - immunology; Neutrophils - pathology; Other diseases. Semiology; Receptors, Leukocyte-Adhesion - immunology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
• ISSN: 0140-6736; 1474-547X
• DOI: 10.1016/S0140-6736(98)05050-8

Endothelial cells that line microvascular blood vessels have an important role in inflammation through their ability to bind and recruit circulating leucocytes. Endothelial cells from the intestines of patients with chronically inflamed Crohn's disease and ulcerative colitis—the two forms of inflammatory bowel disease—display an increased leucocyte-binding capacity in vitro. We investigated whether this enhanced leucocyte binding is a primary or an acquired defect. We cultured human intestinal microvascular endothelial cells (HIMEC) from the uninvolved intestine and chronically inflamed bowel of three patients with inflammatory bowel disease (two Crohn's disease, one ulcerative colitis). We assessed HIMEC binding to polymorphonuclear leucocytes and U937 cells by means of an adhesion assay. After activation with interleukin-1β or lipopolysaccharide, HIMEC from the chronically inflamed tissue in all three patients with inflammatory bowel disease bound twice as many polymorphonuclear leucocytes and U937 cells as endothelial cells from uninvolved tissue. Enhanced leucocyte binding by HIMEC from chronically inflamed tissue in patients with inflammatory bowel disease is an acquired defect since it is not found in the uninvolved intestinal segments from the same individuals. Because interaction between endothelial cells and leucocytes is a key regulatory step in the inflammatory process, this enhanced binding may contribute to the pathophysiology of chronic intestinal inflammation.