Alterations in tumour suppressor gene p53 correlate with inhibition of thrombospondin-1 gene expression in colon cancer cells

If activation of the p53 gene is involved in the progression or metastasis of colon cancer, it may affect the angiogenic phenotype in vivo. To verify this hypothesis, we studied the correlation between p53 accumulation and expression of thrombospondin-1 (TSP1) in colon cancer specimens. Levels of TS...

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Bibliographic Details
Published in:Virchows Archiv : an international journal of pathology 1998-11, Vol.433 (5), p.415-418
Main Authors: TOKUNAGA, T, NAKAMURA, M, TAMAOKI, N, UEYAMA, Y, OSHIKA, Y, TSUCHIDA, T, KAZUNO, M, FUKUSHIMA, Y, KAWAI, K, ABE, Y, KIJIMA, H, YAMAZAKI, H
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Aged
Biological and medical sciences
Blotting, Northern
Colonic Neoplasms - genetics
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
DNA Primers - chemistry
DNA, Neoplasm - analysis
Female
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression Regulation, Neoplastic
Genes, p53 - genetics
Humans
Immunoenzyme Techniques
Male
Medical sciences
Middle Aged
Mutation
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Thrombospondin 1 - genetics
Thrombospondin 1 - metabolism
Tumor Suppressor Protein p53 - metabolism
Tumors
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Summary:If activation of the p53 gene is involved in the progression or metastasis of colon cancer, it may affect the angiogenic phenotype in vivo. To verify this hypothesis, we studied the correlation between p53 accumulation and expression of thrombospondin-1 (TSP1) in colon cancer specimens. Levels of TSP1 gene expression were estimated by Northern blotting in 65 colon cancers. Accumulation of p53 and the distribution of TSP1 protein were evaluated immunohistochemically. Various levels of TSP1 gene expression were seen in colon cancers, while p53 accumulation was confirmed in 42 of the 65 colon cancers. The level of TSP1 gene expression demonstrated a significant inverse correlation with p53 accumulation in colon cancer. Colon cancer cells expressed TSP1 protein and p53 accumulation reciprocally in the same nests. These results suggest that alterations in the tumour suppressor gene p53 may inhibit TSP1 expression in colon cancer.
ISSN:0945-6317
1432-2307
DOI:10.1007/s004280050268