Human D3 dopamine receptor in the medulloblastoma TE671 cell line: cross‐talk between D1 and D3 receptors

In search of a cell line in which the D3 dopamine receptor is expressed endogenously, we found that the neuron‐derived human medulloblastoma cell line TE671 expresses the human D3 (hD3) and D1 (hD1) receptor, but neither the D2 or D4 receptors. Exposure of TE671 cells to the D3 agonist 7‐OH‐DPAT (DP...

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Bibliographic Details
Published in:FEBS letters 1998-11, Vol.439 (1-2), p.138-142
Main Authors: Levavi-Sivan, Berta, Park, Bae-Hang, Fuchs, Sara, Fishburn, C.Simone
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Dopamine receptor
Human
Humans
Medulloblastoma
Mice
Protein Conformation
Receptors, Dopamine D1 - biosynthesis
Receptors, Dopamine D1 - genetics
Receptors, Dopamine D1 - metabolism
Receptors, Dopamine D2 - genetics
Receptors, Dopamine D2 - metabolism
Receptors, Dopamine D3
TE671
Up-Regulation
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Summary:In search of a cell line in which the D3 dopamine receptor is expressed endogenously, we found that the neuron‐derived human medulloblastoma cell line TE671 expresses the human D3 (hD3) and D1 (hD1) receptor, but neither the D2 or D4 receptors. Exposure of TE671 cells to the D3 agonist 7‐OH‐DPAT (DPAT), or to the D1 agonist SKF‐38393 (SKF) increased the expression of hD3 or hD1 mRNA, respectively. Moreover, whereas DPAT had no effect on hD1 mRNA levels, stimulating the cells with SKF caused an increase in both hD1 and hD3 transcript levels. These results suggest (i) that following ligand stimulation, hD3 and hD1 receptors are upregulated to enhance their own receptor expression, and (ii) that upregulation of hD1 receptor transcripts leads to a stimulation of the hD3 dopamine receptor transcripts.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(98)01356-8