Biomolecules-carbon nanotubes doped conducting polymer nanocomposites and their sensor application

A simple method for preparing bio-functionalized soluble single-walled carbon nanotubes (SWNTs) is described. Different proteins such as bovine serum albumin (BSA), cytochrome c and horseradish peroxidase (HRP) were used to solubilize low functionality SWNTs in water aided by sonication. The unbound...

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Bibliographic Details
Published in:Talanta (Oxford) 2007-12, Vol.74 (3), p.370-375
Main Authors: Kum, Maxwell C., Joshi, Kanchan A., Chen, Wilfred, Myung, Nosang V., Mulchandani, Ashok
Format: Article
Language:English
Subjects:
Absorption
Animals
Biomolecule
Biosensing Techniques - instrumentation
Biosensor
Carbon nanotubes
Cattle
Conducting polymer
Cytochromes c
Electrochemistry
Horseradish Peroxidase - chemistry
Horseradish Peroxidase - metabolism
Nanocomposite
Nanocomposites - chemistry
Nanotubes, Carbon - chemistry
Polymers - chemistry
Polypyrrole
Proteins - chemistry
Pyrroles - chemistry
Serum Albumin, Bovine
Solubility
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Summary:A simple method for preparing bio-functionalized soluble single-walled carbon nanotubes (SWNTs) is described. Different proteins such as bovine serum albumin (BSA), cytochrome c and horseradish peroxidase (HRP) were used to solubilize low functionality SWNTs in water aided by sonication. The unbound proteins were removed by column chromatography and the SWNT-protein conjugate was used as the sole anionic dopant in electropolymerization of polypyrrole from polymerization solution at pH above the isoelectric point of the protein to provide a negative charge. The morphology of the polypyrrole with SWNT-protein dopant was found to be three-dimensional and fibrous with wide open interlocking pores in contrast to smooth and cauliflower-like for chloride doped polypyrrole. Enhanced sensor performance was demonstrated for hydrogen peroxide detection on polypyrrole/SWCNT-HRP nanocomposites modified electrode. Such nanocomposites can be potentially applied for other biosensor and bio-fuel cell applications.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2007.08.047