Expression and activity patterns of nitric oxide synthases and antioxidant enzymes reveal a substantial heterogeneity between cardiac and vascular aging in the rat

We investigated the effects of aging and ischemia-reperfusion (I/R) injury on the expression and activity of nitric oxide (*NO) synthases and superoxide dismutase (SOD) isoforms. To this end we perfused excised hearts from young (6 months old) and old (31-34 months old) rats according to the Langend...

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Published in:Biogerontology (Dordrecht) 2005-11, Vol.6 (5), p.325-334
Main Authors: VAN DER LOO, Bernd, BACHSCHMID, Markus, LABUGGER, Ralf, SCHILDKNECHT, Stefan, KILO, Juliane, HAHN, Raphael, PALACIOS-CALLENDER, Miriam, LÜSCHER, Thomas F
Format: Article
Language:English
Subjects:
Aging
Aging - metabolism
Animals
Biological and medical sciences
Femoral Artery - metabolism
Fundamental and applied biological sciences. Psychology
Glutathione - metabolism
Heart
In Vitro Techniques
Ischemia
Isoenzymes - analysis
Male
Myocardial Stunning
Myocardium - metabolism
Nitric oxide
Nitric Oxide Synthase - biosynthesis
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type III - analysis
Nitric Oxide Synthase Type III - metabolism
Perfusion - methods
Rats
Rats, Inbred F344
Renal Artery - metabolism
Rodents
Superoxide Dismutase - analysis
Superoxide Dismutase - metabolism
Tyrosine - analogs & derivatives
Tyrosine - metabolism
Vertebrates: cardiovascular system
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Summary:We investigated the effects of aging and ischemia-reperfusion (I/R) injury on the expression and activity of nitric oxide (*NO) synthases and superoxide dismutase (SOD) isoforms. To this end we perfused excised hearts from young (6 months old) and old (31-34 months old) rats according to the Langendorff technique. The isolated hearts were, after baseline perfusion for 30 min, either subjected to 20 min of global no-flow ischemia followed by 40 min of reperfusion or were control-perfused (60 min normoxic perfusion). Both MnSOD and Cu,ZnSOD expression remained unchanged with increasing age and remained unaltered by I/R. However, SOD activity decreased from 7.55 +/- 0.1 U/mg protein in young hearts to 5.94 +/- 0.44 in old hearts (P
ISSN:1389-5729
1573-6768
DOI:10.1007/s10522-005-4807-1