Mitochondrial DNA content and 4977 bp deletion in unfertilized oocytes

• Published in: Molecular human reproduction 2005-12, Vol.11 (12), p.843-846
• Main Authors: Chan, C.C.W., Liu, V.W.S., Lau, E.Y.L., Yeung, W.S.B., Ng, E.H.Y., Ho, P.C.
• Format: Article
• Language: English
• Subjects: Adult; age; Biological and medical sciences; deletion; DNA, Mitochondrial - genetics; DNA, Mitochondrial - isolation & purification; Embryology: invertebrates and vertebrates. Teratology; Female; Fertilization; Fundamental and applied biological sciences. Psychology; Humans; Infertility, Female - genetics; Menstrual Cycle; mitochondrial DNA copy number; Oocytes - physiology; Polymerase Chain Reaction; Sequence Deletion; unfertilized oocyte
• ISSN: 1360-9947; 1460-2407
• DOI: 10.1093/molehr/gah243

Previous studies analysing the incidences of mitochondrial DNA (mtDNA) deletions and mtDNA content in unfertilized oocytes in relation to donors’ age have been controversial. The objective of the study was to compare these two parameters in unfertilized oocytes and relate them to the donors’ age. Fifty-two women donated 155 unfertilized metaphase II (MII) oocytes. The incidence of 4977 bp deletion was 34.6%, and the mtDNA copy number was 598 350 ± 265 862. Women ≥35 years of age had a significantly higher incidence of 4977 bp deletion, lower mtDNA copy number, higher FSH level and poorer ovarian response when compared with younger women. The mtDNA copy number was negatively correlated with the donor’s age. The higher incidence of mtDNA deletion and lower mtDNA copy number in older women suggested that these two parameters may reflect ovarian ageing.