Exophiala dermatitidis and Sarcinomyces phaeomuriformis : ITS1-sequencing and nutritional physiology

The internal transcribed spacer 1 (ITS1) sequences of the nuclear rRNA gene (approximately 200 bp) of 33 strains of the Exophiala dermatitidis complex were determined; two similar species were added for comparison. A core group (I), including the type strain CBS 207. 35, contained 20 identical strai...

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Bibliographic Details
Published in:Medical mycology (Oxford) 1998-06, Vol.36 (3), p.143-151
Main Authors: UIJTHOF, J. M. J, VAN BELKUM, A, DE HOOG, G. S, HAASE, G
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
DNA, Fungal - genetics
DNA, Ribosomal - genetics
Exophiala - classification
Exophiala - genetics
Exophiala - physiology
Exophiala dermatitidis
Fundamental and applied biological sciences. Psychology
Genes. Genome
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Microbiology
Mitosporic Fungi - classification
Mitosporic Fungi - genetics
Mitosporic Fungi - physiology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mycology
Phylogeny
Restriction Mapping
RNA, Fungal - genetics
RNA, Ribosomal, 5.8S - genetics
Sequence Alignment
Sequence Homology, Nucleic Acid
Species Specificity
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Summary:The internal transcribed spacer 1 (ITS1) sequences of the nuclear rRNA gene (approximately 200 bp) of 33 strains of the Exophiala dermatitidis complex were determined; two similar species were added for comparison. A core group (I), including the type strain CBS 207. 35, contained 20 identical strains which had previously been found to have introns in their small sub-unit (SSU) rDNA. Eleven remaining strains identified as E. dermatitidis (groups II-V) differed from the core group in 1-4 nucleotide positions (plus a deletion in one strain); most of them lacked introns in their SSU ribosomal genes. The type strain of the meristematic species Sarcinomyces phaeomuriformis CBS 131.88 was found to differ significantly from E. dermatitidis. One strain had the annellidic morphology of E. dermatitidis, but the ITS1 sequence of S. phaeomuriformis. Strain CBS 709.95, an E. dermatitidis reported to have a meristematic synanamorph, was found to have ITS1 identity to the type strain of E. dermatitidis, although SSU sequences established previously suggested a close relationship with S. phaeomuriformis. Slight physiological differences were found between E. dermatitidis and S. phaeomuriformis. An oligonucleotide probe specific for E. dermatitidis was designed, thus able to discriminate this species from closely related black yeasts.
ISSN:1369-3786
1460-2709
DOI:10.1046/j.1365-280X.1998.00143.x