Macrophage-colony stimulating factor enhances MHC-restricted presentation of exogenous antigen in dendritic cells

Previous studies have shown that dendritic cells (DCs) can phagocytize, process and present a microencapsulated form of ovalbumin (OVA) in the context of class I MHC as well as class II MHC. In the present study, we examined the effects of recombinant human macrophage-colony stimulating factor (M-CS...

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Published in:Cytokine (Philadelphia, Pa.) Pa.), 2005-12, Vol.32 (5), p.187-193
Main Authors: Han, Shinha, Song, Youngcheon, Lee, Young-Hee, Lee, Young-Ran, Lee, Chong-Kil, Cho, Kyunghae, Kim, Kyungjae
Format: Article
Language:English
Subjects:
Animals
Antigen presentation
Antigen Presentation - drug effects
Antigens - immunology
Antigens - metabolism
Bone Marrow Cells - immunology
Cell Line
Cross-Priming
Dendritic cell
Dendritic Cells - immunology
Dendritic Cells - metabolism
Histocompatibility Antigens Class II - immunology
Histocompatibility Antigens Class II - metabolism
Humans
Macrophage Colony-Stimulating Factor - pharmacology
Macrophage-colony stimulating factor
Mice
Ovalbumin - immunology
Ovalbumin - metabolism
Phagocytosis - drug effects
Phagocytosis - immunology
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Summary:Previous studies have shown that dendritic cells (DCs) can phagocytize, process and present a microencapsulated form of ovalbumin (OVA) in the context of class I MHC as well as class II MHC. In the present study, we examined the effects of recombinant human macrophage-colony stimulating factor (M-CSF) on the MHC-restricted presentation of microencapsulated OVA by DCs. Two types of DCs were generated from mouse bone marrow (BM) cells, one type with granulocyte/macrophage-colony stimulating factor (GM-CSF) alone, the other type with GM-CSF and interleukin (IL)-4. Pretreatment with M-CSF significantly enhanced both class I MHC and class II MHC-restricted presentation of exogenous OVA by both types of DCs. The enhancing activity of M-CSF on antigen presentation was more potent in DCs generated with GM-CSF alone compared to DCs generated with both GM-CSF and IL-4. Pretreatment of the DCs with M-CSF did not increase phagocytic activity or total level of expression of class I MHC (H-2K b) molecules, but increased expression of OVA peptide-H-2K b complexes upon phagocytosis of microencapsulated OVA. These results demonstrate that M-CSF increases intracellular processing events of phagocytized antigen in DCs.
ISSN:1043-4666
1096-0023
DOI:10.1016/j.cyto.2005.08.002