New fluorescent labels: 4- and 7-chlorofluorescein

A new mixture of 4- and 7-chlorofluorescein were synthesized by condensation of resorcinol with 3-chlorophthalic anhydride in the presence of methanesulfonic acid or zinc chloride. These regioisomers were successfully separated by chromatography. The photophysical properties were examined and their...

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Bibliographic Details
Published in:Journal of fluorescence 2005-11, Vol.15 (6), p.829-833
Main Authors: Ge, Feng-Yan, Yan, Xi-Long, Yan, Fan-Yong, Pan, Hui-Ying, Chen, Li-Gong
Format: Article
Language:English
Subjects:
Fluoresceins - chemical synthesis
Fluoresceins - chemistry
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Hydrogen-Ion Concentration
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Summary:A new mixture of 4- and 7-chlorofluorescein were synthesized by condensation of resorcinol with 3-chlorophthalic anhydride in the presence of methanesulfonic acid or zinc chloride. These regioisomers were successfully separated by chromatography. The photophysical properties were examined and their absorption and emission maxima at long wavelength, high fluorescence quantum yield, and narrow emission bandwidth were found, highly favorable for detecting multiple target substances in the same sample. Furthermore, 4(7)-chlorofluorescein was found to be strongly pH-dependent between 4.0 and 8.0, and could be used as pH-sensitive fluorescent probe to measure intracellular pH.
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-005-0016-1