Investigation of Mycoplasma agalactiae in milk and conjunctival swab samples from sheep flocks in west central, Iran

In a study to investigate the current status of Mycoplasma agalactiae infections in sheep flocks in west central, Iran, a total of 54 and 47 conjunctival swab and milk samples were collected from normal healthy ewes of 26 herds, respectively. Using PCR method. Mycoplasma agalactiae detected in 20 ou...

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Bibliographic Details
Published in:Pakistan journal of biological sciences 2007-04, Vol.10 (8), p.1346-1348
Main Authors: Kheirabadi, K H Pirali, Ebrahimi, A
Format: Article
Language:English
Subjects:
Animals
Conjunctiva - microbiology
DNA Primers
Female
Milk - microbiology
Mycoplasma agalactiae
Mycoplasma agalactiae - isolation & purification
Mycoplasma Infections - diagnosis
Mycoplasma Infections - veterinary
Polymerase Chain Reaction
Reference Values
RNA, Bacterial - genetics
RNA, Bacterial - isolation & purification
RNA, Ribosomal, 16S - genetics
RNA, Ribosomal, 16S - isolation & purification
Sheep
Sheep Diseases - microbiology
Surveys and Questionnaires
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Summary:In a study to investigate the current status of Mycoplasma agalactiae infections in sheep flocks in west central, Iran, a total of 54 and 47 conjunctival swab and milk samples were collected from normal healthy ewes of 26 herds, respectively. Using PCR method. Mycoplasma agalactiae detected in 20 out of 101 animals (19.8%) examined, 12 out of 54 (22.2%) of conjunctival swab and 8 out of 47 (17%) milk samples were positive. Samples of 20 (out of 26) herds were positives, that means 77% of sheep herds in this region have at least one ewes infected by M. agalactiae. The results confirmed that herds in west central Iran have currently about 20% infection rates for contagious agalactiae of sheep.
ISSN:1028-8880
DOI:10.3923/pjbs.2007.1346.1348