Spasmolytic effect of Petroselinum crispum (Parsley) on rat's ileum at different calcium chloride concentrations

Traditional herbal medicines such as Petroselinum crispum (Parsley) have been used for treatment of intestinal disorders in Iran. To date no pharmacological evidence for their effectiveness has been reported. The aim of this study was to examine the relaxant effect of hydroalcoholic extract of Petro...

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Bibliographic Details
Published in:Pakistan journal of biological sciences 2007-11, Vol.10 (22), p.4036-4042
Main Authors: Moazedi, A A, Mirzaie, D N, Seyyednejad, S M, Zadkarami, M R, Amirzargar, A
Format: Article
Language:English
Subjects:
Animals
Calcium Chloride - pharmacology
Dose-Response Relationship, Drug
Hydrogen-Ion Concentration
Ileum - drug effects
Male
Oscillometry
Parasympatholytics - pharmacology
Petroselinum - metabolism
Plant Extracts - pharmacology
Potassium Chloride
Rats
Rats, Wistar
Temperature
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Summary:Traditional herbal medicines such as Petroselinum crispum (Parsley) have been used for treatment of intestinal disorders in Iran. To date no pharmacological evidence for their effectiveness has been reported. The aim of this study was to examine the relaxant effect of hydroalcoholic extract of Petroselinum crispum on isolated adult male Wistar rat's ileum contraction by KCl 60 (mM). The extract was prepared from parsley seed with 80% ethanol. A portion of ileum was removed and placed in an organ bath containing Tyrode solution (37 degrees C, pH = 7.4) bubbled with air. From a resting tension of 1 g, isotonic Transducer and Harvard Universal Oscillograph were used for recording contraction of ileum after administration of 60 mM KCl. Various concentration of extract were added to the bath. This experiment showed that, the extract in a dose-dependent manner decreased the induced-KCl ileums contraction (n = 7, p < 0.001). Also adding extract before influence of KCl, cause reduced effect of KCl (n = 7, p < 0.001). In addition, the Tyrode solution without CaCl2 and high concentration of K+, various concentration of extract significantly (n = 7, p < 0.001) decreased the CaCl2-induced contraction. Therefore, it seems that the relaxation effect of extract alcoholic parsley seed on the contraction of ileum is performed by blocking of voltage-gated calcium channels.
ISSN:1028-8880
DOI:10.3923/pjbs.2007.4036.4042