A simple and rapid assay for specific identification of bovine derived products in biocomplex materials

A simple and rapid method for specific identification of beef or bovine-derived products in processed food and in animal feed concentrates was developed and evaluated using Polymerase Chain Reaction (PCR). The mitochondrial cytochrome-b (mtcyt-b) gene was used as a target DNA for PCR amplification....

Full description

Saved in:
Bibliographic Details
Published in:Pakistan journal of biological sciences 2007-04, Vol.10 (8), p.1170-1174
Main Authors: Khairalla, Khairalla M S, Aradaib, Imadeldin E, Bakhiet, Amel O, Hassan, Tigani, Hago, Badr E, Saeed, Abdel-Rahman
Format: Article
Language:English
Subjects:
Animal Feed - standards
Animals
Cattle
Cytochromes b - genetics
DNA - genetics
DNA - isolation & purification
DNA Primers
Meat - analysis
Mitochondria - genetics
Mitochondria, Muscle - enzymology
Mitochondria, Muscle - genetics
Polymerase Chain Reaction - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A simple and rapid method for specific identification of beef or bovine-derived products in processed food and in animal feed concentrates was developed and evaluated using Polymerase Chain Reaction (PCR). The mitochondrial cytochrome-b (mtcyt-b) gene was used as a target DNA for PCR amplification. Three primers derived from a highly conserved region of bovine mtcyt-b gene were used. The outer pair of primers (RSL1 and CSR2) produced a 365 base pair (bp) PCR ampilicon from bovine DNA, while the internal semi-nested pair of primers (CSL1 and CSR2) were used to amplify a 284 bp PCR ampilicon, internal to the annealing sites of primers (RSL1 and CSR2). Both ampilicons were identified easily after visualization on agarose gel stained with ethidium bromide. The specificity studies indicated that the primary or the semi-nested PCR products were not amplified from DNA extracted from different ruminant species including, sheep, goat and ghazals; or from non-ruminant animals including camels, horses and pigs. Also was found very sensitive because could detect 0.001% (W/V) of bovine mtcyt-b gene. The semi-nested amplification was necessary to increase the sensitivity of the PCR assay and to confirm the identity of the primary PCR ampilicons. The described PCR assay detected the primary and the semi-nested PCR ampilicons from different animal feed concentrates containing bovine-derived product including, canned food, poultry and dairy feed concentrates. The described PCR assay should facilitate rapid detection of beef and bovine-derived products in processed food and in animal feed concentrates.
ISSN:1028-8880
DOI:10.3923/pjbs.2007.1170.1174