Mannosylated semiconductor quantum dots for the labeling of macrophages

Quantum dots show strong fluorescence emission and long stability compared with classical organic fluorescent dyes; therefore, quantum dots take the place of other dyes in Western blot, immunostaining and bioimaging. Since macrophage plays crucial roles in many pathophysiological processes, tracking...

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Bibliographic Details
Published in:Journal of controlled release 2008-01, Vol.125 (2), p.131-136
Main Authors: Higuchi, Yuriko, Oka, Machiko, Kawakami, Shigeru, Hashida, Mitsuru
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line, Tumor
Cells, Cultured
Endocytosis
Female
General pharmacology
Humans
Imaging
Lectins, C-Type - metabolism
Macrophage
Macrophages, Peritoneal - metabolism
Mannose - chemistry
Mannose - metabolism
Mannose-Binding Lectins - metabolism
Mannosylation
Medical sciences
Mice
Mice, Inbred ICR
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Polyethylene Glycols - chemistry
Polyethylene Glycols - metabolism
Quantum dot
Quantum Dots
Receptor-mediated endocytosis
Receptors, Cell Surface - metabolism
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Summary:Quantum dots show strong fluorescence emission and long stability compared with classical organic fluorescent dyes; therefore, quantum dots take the place of other dyes in Western blot, immunostaining and bioimaging. Since macrophage plays crucial roles in many pathophysiological processes, tracking macrophage migration, homing and fate is important for understanding the complex roles of macrophages in disease or developing disease diagnosis. Because of the high expression of mannose receptor on macrophage, mannosylation is an attractive strategy to label macrophage. In this study, using polyethylene-glycol (PEG) (M.W. 2,000; PEG 2,000)-coated quantum dots, we prepared mannosylated PEG 2,000 (Man-PEG 2,000) quantum dots for labeling macrophage. The uptake characteristics of Man-PEG 2,000 quantum dots were investigated by primary cultured peritoneal macrophages. The uptake of Man-PEG 2,000 quantum dots was inhibited by an excess amount of mannose, suggesting mannose receptor-mediated uptake of Man-PEG 2,000 quantum dots. The result of MTT assay suggested the extremely low cytotoxicity of Man-PEG 2,000 quantum dots. In conclusion, the Man-PEG 2,000 synthesized is safe and is taken up by macrophage mannose receptor recognition.
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2007.10.007