Quantitative determination of drug encapsulation in poly(lactic acid) nanoparticles by capillary electrophoresis

Capillary electrophoretic (CE) methods were used for the quantitative determination of model drugs [salbutamol sulphate (SS), sodium cromoglycate (SCG) and beclomethasone dipropionate (BDP)] in poly( d,l-lactic acid) (PLA) nanoparticles, which were prepared by the nanoprecipitation method. Zeta pote...

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Bibliographic Details
Published in:Journal of Chromatography A 2008-01, Vol.1178 (1), p.248-255
Main Authors: Helle, Anne, Hirsjärvi, Samuli, Peltonen, Leena, Hirvonen, Jouni, Wiedmer, Susanne K.
Format: Article
Language:English
Subjects:
Albuterol - analysis
Analysis
Beclomethasone - analysis
Biological and medical sciences
Capillary electrophoresis
Cromolyn Sodium - analysis
Drug Carriers - analysis
Drug loading
Electrophoresis, Capillary
General pharmacology
Lactic Acid
Medical sciences
Microemulsion
Microscopy, Electron, Scanning
Nanoparticles
Nanoparticles - analysis
Nanoparticles - ultrastructure
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - isolation & purification
Pharmacology. Drug treatments
Poly( d,l-lactic acid)
Polyesters
Polymers
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Summary:Capillary electrophoretic (CE) methods were used for the quantitative determination of model drugs [salbutamol sulphate (SS), sodium cromoglycate (SCG) and beclomethasone dipropionate (BDP)] in poly( d,l-lactic acid) (PLA) nanoparticles, which were prepared by the nanoprecipitation method. Zeta potential and size distribution of the nanoparticles were determined by electrophoretic mobility determinations and photon correlation spectroscopy, respectively. Interactions between the drugs, the PLA nanoparticles and the fused-silica capillary were investigated by electrokinetic capillary chromatography (EKC). A quantitative CE method was developed for salbutamol sulphate and sodium cromoglycate, and the linearity and repeatability of migration times, peak areas and peak heights were determined. Microemulsion electrokinetic chromatography was used for the quantitative determination of beclomethasone dipropionate. According to this study, the applied electromigration techniques were suitable for the interaction, drug entrapment and dissolution studies of pharmaceutical nanoparticles. The results suggest that even quantitation of the drug located inside the nanoparticles was possible. Encapsulation of the more hydrophilic model drugs (SS, SCG) in the PLA nanoparticles was less efficient than in the case of BDP.
ISSN:0021-9673
DOI:10.1016/j.chroma.2007.11.041