Interaction of rifabutin with model membranes

Liposomal and free rifabutin were separated by the method of gel filtration. The percents of rifabutin bound to liposomes of different phospholipid composition were measured. The presence of negatively charged phospholipids increased the degree of binding. Binding decreased with increasing the ionic...

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Published in:Bulletin of experimental biology and medicine 2005-12, Vol.140 (6), p.711-713
Main Authors: Shakina, Yu N, Vostrikov, V V, Sorokoumova, G M, Selishcheva, A A, Shvets, V I
Format: Article
Language:English
Subjects:
Adsorption
Animals
Antibiotics, Antitubercular - pharmacology
Chromatography, Gel
Ions
Lipid Bilayers - metabolism
Liposomes - chemistry
Membranes - chemistry
Phosphatidylcholines - chemistry
Protein Binding
Rifabutin - chemistry
Rifabutin - pharmacology
Spectrometry, Fluorescence
Static Electricity
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cited_by cdi_FETCH-LOGICAL-c326t-ce670c9a224ed6f050523e93ba97d836818d96cabe4d6104d9bb6c49f4efb4473
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creator Shakina, Yu N
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Shvets, V I
description Liposomal and free rifabutin were separated by the method of gel filtration. The percents of rifabutin bound to liposomes of different phospholipid composition were measured. The presence of negatively charged phospholipids increased the degree of binding. Binding decreased with increasing the ionic strength. Incubation of rifabutin with liposomes containing anthryl phosphatidylcholine was accompanied by fluorescence quenching. Activity of rifabutin depended on the phospholipid composition of liposomes. Our results indicate that binding of rifabutin is associated with electrostatic and hydrophobic interactions.
doi_str_mv 10.1007/s10517-006-0062-y
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subjects Adsorption
Animals
Antibiotics, Antitubercular - pharmacology
Chromatography, Gel
Ions
Lipid Bilayers - metabolism
Liposomes - chemistry
Membranes - chemistry
Phosphatidylcholines - chemistry
Protein Binding
Rifabutin - chemistry
Rifabutin - pharmacology
Spectrometry, Fluorescence
Static Electricity
title Interaction of rifabutin with model membranes
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