Fast Determination of Salicylic Acid in Pharmaceuticals by Using a Terbium-Sensitized Luminescent SIA Optosensor

In this article, we report the coupling of sequential injection analysis (SIA) and solid phase lanthanide-sensitized luminescence as a detection technique; in this technique, the energy absorbed by the analyte (retained on a solid support) is transferred to the lanthanide ion, which finally emits th...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 2008-02, Vol.97 (2), p.791-797
Main Authors: Llorent-Martínez, E.J., Domínguez-Vidal, A., Ortega-Barrales, P., Molina-Díaz, A.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Flow Injection Analysis - methods
flow-through optosensor
General pharmacology
Luminescent Measurements
Medical sciences
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
salicylic acid (SA)
Salicylic Acid - analysis
sequential injection analysis (SIA)
Terbium
terbium-sensitized luminescence
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Summary:In this article, we report the coupling of sequential injection analysis (SIA) and solid phase lanthanide-sensitized luminescence as a detection technique; in this technique, the energy absorbed by the analyte (retained on a solid support) is transferred to the lanthanide ion, which finally emits the luminescence signal. By using this automatic system, the determination of salicylic acid (SA) is easily, rapidly, and selectively achieved. Microspheres of commercial solid support, Sephadex-QAE A-25, are used to fill the flow-through cell and retain analyte and terbium ions; after the signal from SA–terbium complex is obtained, the solid support is easily regenerated by using an ethylenediaminetetraacetic acid disodium salt 2-hydrate (EDTA) solution. Over 200 determinations can be carried out without replacing the resin microbeads. The proposed method shows a 0.045 µg mL−1 detection limit, with an R.S.D. lower than 3% and a sampling frequency up to 30 samples per hour. The system has been applied to the determination of SA in pharmaceuticals obtained from the Spanish Pharmacopoeia, with satisfactory results; an additional recovery study has been carried out with recoveries close to 100%. © 2007 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 97:791–797, 2008
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.21013